应激性高血压局灶性脑缺血大鼠脑组织中性鞘糖脂含量的变化及外源性神经节苷脂GM_1的影响

Changes of neutral glycosylsphingolipid in stress-induced hypertensive rats after focal cerebral ischemia and the study of exogenous GM_1 ganglioside effect on the ischemia

目的研究局灶性脑缺血后脑组织内中性鞘糖脂含量的变化及外源性神经节苷脂GM1对其影响。方法采用雄性SD大鼠,通过足底电刺激方法制备应激性高血压动物模型,在此基础上阻断一侧大脑中动脉,制成局灶性脑缺血。实验组于缺血前30min腹腔内注射GM1(10mg／kg体重)。分别测定缺血后3、12、24h后脑组织内中性鞘糖脂的含量。结果在缺血的早期中性鞘糖脂的含量明显降低,随着缺血时间的延长其含量逐渐升高。GM1保护组中性鞘糖脂含量的变化明显轻于实验组。结论脑缺血可以导致神经质膜结构成分发生变化而神经节苷脂GM1对脑缺血具有脑保护作用。

Objective Studies the change of neutral glycosylsphingolipid after rats left MCAO and exogenous GM1ganglioside on brain ischemia. Methods Male rats stress-induced hypertensive model were been performed by repetitive foot electrosock with noise stimulation. After that the rats left middle common artery were occlude. In drug group GM1（10mg/kg. wt） were administered by intraperitoneal injection before MCAO 30min. Neutral glycosylsphingolipid were detected after MCAO 3,12,24h both in control and in GM1 group. Results In the early stage of ischemia the neutral glycosylsphingolipid decreased,with the lasting of ischemia the contents of neutral glycosylsphingolipid increased in ischemic brain. In GM1group neutral glycosylsphingolipid changes were not severity compared with control. Conclusion The results suggest that neuron membrane structural component have changed during acute focal cerebral ischemia but GM1 might be more resistant to ischemia.