脂联素基因SNP276多态性与冠心病及胰岛素敏感性的相关性

Relations of Adiponectin Gene SNP276 With Coronary Heart Disease and Insulin Sensitivity

目的:观察中国人脂联素基因(apM1)与冠心病(CHD)及胰岛素敏感性的关系。方法:对照组136例,冠心病患者281例,又分为单纯CHD患者131例,合并2型糖尿病患者150例,均为无亲缘关系的安徽地区汉族人。用稳态模式胰岛素抵抗指数(HOMA-IR)及胰岛β细胞功能指数(HOMA-β)估测胰岛β细胞胰岛素分泌功能及外周组织胰岛素敏感性。采用聚合酶链反应-单链构象多态性(PCR-SSCP)方法初筛apM1基因后,进行DNA直接测序。结果:apM1基因SNP276基因型GG+CT与TT型相比患CHD的风险增加,风险比值(OR)=2.976,95%可信限1.135-7.804,P=0.027,在固定性别、年龄及体重指数后,偏相关分析表明,GG+CT基因型与HOMA-IR显著正相关(r=0.174,P=0.005)。对照组中GG型或GT型与TT型比较,GG型或GT型的体重指数、腰臀比、体脂百分比、收缩压、空腹胰岛素及HOMA-IR均高于TT型(P<0.05,0.01或0.001),同时,GG型的空腹血糖亦显著升高(P<0.05)。结论:apM1基因SNP276多态性与冠心病及胰岛素敏感性具有相关性。

Objective： To investigate the impact of adiponectin gene SNP276 on coronary heart disease（CHD） and insulin sensitivity in Chinese Hans. Methods： The study population included 136 controls and 281 CHD patients in Anhui Province. The islet beta-cell insulin secretion and tissue insulin sensitivity were assessed by formulae of homeostasis model assessment（ HOMA-IR and HOMA-β）. Firstly, polymerase chain reaction-single strand conformation polymorphism （PCR-SSCP） was used to determine whether variation existed in apM1 gene. Then, exact variation was performed by automated DNA direct sequencing. Results： ①The SNP276 non-TT（GT ＋ GG） genotype was associated with increased risk of CHD （OR = 2.976,95% CI 1. 135-7. 804,P = 0. 027）. After adjusting for sex, age and body mass index（BMI）, non-TT genotype was markedly positively correlated with HOMA-IR（r = 0. 174, P = 0. 005）. ②In controls, the subjects with SNP276 GG or GT genotype had a higher BMI, waist/hip ratio, body fat content, systolic blood pressure, fasting plasma insulin and HOMA-IR than those with TT genotype （P 〈 0. 05, 0.01 or 0. 001 ）. Besides, GG genotype had higher fasting plasma glucose（P 〈 0. 05）. Conclusion： apM1 gene SNP276 was associated with CHD and insulin sensitivity.