急性冠状动脉综合征患者树突状细胞介导的热休克蛋白60特异性细胞毒作用

Dendritic Cell Mediated Heat-Shock Protein 60 Specific T Cytotoricity in Acute Coronary Syndrome

目的:探讨急性冠状动脉综合征(ACS)患者体内是否存在树突状细胞(DC)介导的热休克蛋白60(HSP60)特异性的T细胞毒反应。方法:分离ACS患者(ACS组),稳定性心绞痛(SA)患者(SA组)和正常人(对照组)外周血单核细胞,制备DC。流式细胞仪检测HSP60负载及未负载的DC CD86的表达及各组T细胞中CD45RO+细胞的百分比;二氮唑法(MTT)法检测各组DC刺激自体淋巴细胞增殖的能力,并检测各组DC诱导的细胞毒T细胞(CTL)对负载HSP60的人脐静脉内皮细胞(HUVECS)的杀伤活性;酶联免疫吸附法(ELISA)测定HSP60冲击T细胞后干扰素γ的分泌。结果:与未负载HSP60的SA组和对照组的DC比较,ACS组的DC以及负载HSP60对照组的DC CD86表达较高;ACS 组的DC及HSP60负载的DC刺激自体细胞毒T细胞增殖的能力增强;ACS组的DC及HSP60负载的DC诱导的细胞毒T 细胞对负载HSP60的人脐静脉内皮细胞有高度的杀伤作用;ACS患者体内存在HSP60致敏的记忆性T细胞。结论:ACS患者体内存在强烈的DC介导的HSP60抗原特异性T细胞毒反应。

Objective：To investigate whether dendritic cells（DC） derived from patients with acute coronary syndrome （ACS） can induce heat-shock protein 60（HSP60） specific T cell immune response to endothelial cells. Methods ： DCs generated from peripheral blood mononuclear cells of 20 patients with ACS, 15 patients with stable angina and 10 healthy subjects were investigated, CD86 expression on HSP60-pulsed and no-pulsed DCs, and percentage of CD45RO^＋ T cells were detected by flow cytometry. MTI＂ was used to assay the ability of DCs from each group to stimulate autologous T cell proliferation and cytotoxic T lymphocytes（CTL） mediated lysis of HSP60 loaded endothelial cells. ELISA was used to assay interferin-γ release by T cells. Results： Compared with DCs from stable angina and healthy subjects, CD86 was much more expressed in DCs from ACS and from DCS pulsed with HSP60. Capacity of DCs from ACS and from DCs pulsed with HSP60 to stumulate autologous T cell proliferation was increased; DCs from ACS and pulsed with HSP60 could induce HSP60 specific CTLs which could efficiently kill endothelial cells loading HSP60. Percentage of HSP60 specific CD45RO^＋ memory T cells was higher in ACS. Conclusion： There exists DC- mediated HSP60 specific T cell cytotoxity in acute coronary syndrome.