黏附分子ICAM-1基因C469T多态性与冠心病的关系

The study of intercellular adhesion molecule-1 gene polymorphisms C469T in patients with coronary heart disease

目的:探讨中国湖北地区汉族人群黏附分子ICAM-1基因外显子6,469编码处碱基C/T多态性与冠心病(CHD)的关系,以了解CHD发生、发展的遗传机制,通过黏附分子ICAM-1基因型筛选冠状动脉粥样硬化易患人群和CHD患者中高危人群的可能性.方法:①共入选CHD患者145例,其中73例为急性冠状动脉综合征(ACS),72例为稳定型CHD(SCHD),同时入选心电图检查无异常或冠状动脉造影正常者144例为对照组.所有CHD患者均经冠状动脉造影证实.②采用聚合酶链反应-限制性片段长度多态性(PCR-RFLP)技术,分析CHD患者和对照组的ICAM-1基因型.结果:CHD组与对照组及CHD组中ACS者与SCHD者ICAM-1基因C469T 编码处T等位基因频率的差异均有统计学意义(CHD组比对照组χ2=20.254,P<0.01;ACS比SCHD者χ2=3.981,P<0.05 ).基因型频率的相对风险分析发现,TT加CT基因型患CHD的风险是CC基因型的5.288倍(OR=5.288,95%,CI:2.977～9.395).结论:ICAM-1基因C469T多态性与CHD密切相关, T等位基因可能是CHD发病的遗传易感基因.

Objective：To explore whether ICAM-1 gene single nucleotide polymorphisms C/T in exon 6 codon 469 related to CHD in Chinese Han peoples of Hubei province. To study the correlation between the different genotypes. Method：The genotypes of ICAM-1 were detected by polymerase chain reaction-restriction fragment length polymorphism （PCR-RFLP） methods in 145 CHD including 73 ACS patients and 72 SCHD patients, and 144 controls. Result：There was significant difference in frequencies of allele in C469T polymorphism between CHD and control group as well as ACS and SCHD respectively （CHD and control group： X^2= 20. 254, P 〈0. 001 ; ACS and SCHD： X^2= 3. 981, P 〈0.05）. The relative risk suffering from CHD with genotype TT＋CT was 5. 288 times of those with genotype CC （ OR = 5. 288, 95%; CI ： 2. 977 - 9. 395）. Conclusion： ICAM-1 gene polymorphyism C469T is significantly associated with the CHD. T allele of ICAM-1 may be a genetic factor that may determine an individual＇s susceptibility for CHD.