卡维地洛抗豚鼠再灌注早期心室颤动作用及其机制

Effect and mechanism of carvedilol on ventricular fibrillation at early phase of reperfusion in guinea pig

目的:探讨卡维地洛抗豚鼠再灌注早期心室颤动作用及其机制。方法:利用心肌缺血再灌注损伤模型,研究卡维地洛在0·1、1·0和10·0μmo1/L3个浓度时对再灌注早期心脏去甲肾上腺素(NE)释放及心室颤动发生的影响。结果:①卡维地洛在1·0μmo1/L和10·0μmo1/L时心脏流出液中的NE含量分别为(13·390±7·011)pmol/g心脏组织和(5·004±2·598)pmol/g心脏组织,均显著低于空白对照组(38·670±13·661)pmol/g(P<0·05);②卡维地洛可明显降低再灌注早期心室颤动的发生。结论:缺血再灌注心脏释放的NE在再灌注心律失常的发生中可能起着重要作用,卡维地洛呈浓度依赖性的减少再灌注早期心脏交感神经递质NE释放的作用应是其抗再灌注心律失常心室颤动的作用机制之一。

Objective：To study the effect and mechanism of carvedilol on ventricular fibrillation at early phase of reperfusion in guinea pig. Method： The model of heart reperfusion injury was used to investigate the effect of 0.1.1.0 and 10.0μmol/L carvedilol on cardiac norepinephrine release and ventricular fibrillation at the early phase of reperfusion. Result：①In hearts perfused with 1.0/μmol/L and 10.0 μmol/L carvedilol, the amounts of norepinephrine release were （ 13.390 ± 7. 011 ） pmol/g and （ 5. 004± 2.598） pmol/g, respectively. There were significant differences （all P 〈0.05） when compared with the control group（38. 670 ±13. 661）pmol/g . ②Carvedilol reduced the incidence of ventricular fibrillation significantly （ P 〈0.05）. Conclusion：h is suggested that released norepinephrine probably plays an important role in the genesis of reperfusion-induced ventricular fibrillation. The decreasing of norepinephrine release by carvedilol contributes to its antiventricular fibrillation effect at the early phase of reperfusion,in a concentration dependent manner.