缺血预处理对大鼠心肌中过氧化物酶体增殖物激活受体α表达的影响

Ischemic preconditioning effects expression of peroxisome proliferator-activated recptorαin ischemia reperfusion myocardium in vivo

目的:探讨缺血预处理(IPC)对实验性大鼠缺血再灌注(IR)心肌梗死(MI)面积及过氧化物酶体增殖物激活受体α(PPARα)表达的影响。方法:Wistar大鼠70只随机分为3组:对照组(24只),IR组(24只),IPC组(22只)。采用鼠心冠状动脉左前降支结扎法制作体内IR模型,观察MI范围,运用半定量RT-PCR方法对心肌PPARαmRNA的表达情况进行分析,运用Western-blotting方法检测PPARα蛋白表达情况。结果:IPC组梗死面积显著减少(P<0·05),并且心肌PPARαmRNA及蛋白表达水平显著上调(P<0·05)。结论:IPC可显著减少实验性大鼠IR MI面积及并使心肌中PPARα显著上调。

Objective：To investigate the effect of preconditioning on infarct size and expression of PPARα in myocardial ischemia/ reperfusion （IR） in vivo. Method： Rats were randomly divided into three groups： the IR group （ n=24） ; the control group （ n=24） ;the ischemic preconditioning group（ IPC, n=22）. Models of IR and IPC were produced by ligating left descending of coronary artery in Wistar rats. Infarct size was documented. PPARα mRNA and protein were analysed by semi quantitive RT-PCR and Western-blotting. Result： Precondition ing significantly reduced myocardial infarct size （ P 〈0.05）. The PPARα mRNA expression and protein of IPC group were much more higher than that in the IR group（ P 〈0.05）. Conclusion：Preconditioning significantly reduces myocardial infarct size, up-regulates mRNA expression and protein level of myocardial PPARα of rats.