CO_2气腹对结肠癌细胞粘附能力影响及其与粘附分子的关系

Effect of CO_(2)-insufflation pressure on the adhesion potential of colon cancer cells and its relationship with expression of adhesion molecules.

目的分析不同压强CO2气腹对结肠癌细胞表面粘附分子表达的影响以及在此基础上对癌细胞粘附能力的影响。方法建立体外气腹模型,选用人结肠癌细胞株SW 1116,分别在6mmHg(1mmHg=0.133 kPa)、9mmHg、12mmHg和15mmHg压强CO2以及常规条件下暴露1h后,流式细胞技术(FACScan)检测粘附分子上皮钙黏着素(E-cadherin)、细胞间粘附分子-1(ICAM-1)、CD44和E-选择素(E-selectin)于处理后0h、12h、24h、48h和72h在结肠癌细胞表面的表达;取各组处理后0 h和72 h细胞进行体外粘附试验。结果经各组压强CO2气腹处理后,SW 1116表面各粘附分子的表达与处理前相比变化有显著性(P<0.05),这些变化在各压强CO2处理后72h之前均恢复至处理前水平,或低于处理前水平(P<0.05)。在处理后0h,随CO2气腹压强增高,E-cadherin、CD44和ICAM-1表达量降低,差异均有显著性(P<0.05),结肠癌细胞的粘附能力也呈下降的趋势(P<0.05),处理后72 h,各压强组之间的细胞粘附差异无显著性。结论CO2气腹对结肠癌细胞表面粘附分子的表达可产生一过性双向影响,随CO2气腹压强的增高,癌细胞粘附分子的表达与粘附能力均受到抑制。

Objective To investigate the influence of CO2-insufflation and its pressure on adhesion potential of the colon cancer cells based on expression of adhesion molecules. Methods With an artificial pneumoperitoneum modal in vitro, SW1116 human colon carcinoma cells were exposed to CO2-insufflation in 5 different pressure groups：6mmHg,9mmHg, 12mmHg, 15mmHg and control group, respectively for 1 hour. Expression of E-cadherin, ICAM-1, CD44 and E-selectin were measured at 0, 12,24,48 and 72 hours after CO2-insufflation by using flowcytometry. The adhesion capacity of SW1116 cells before and after exposed to CO2-insufflation was detected by cell adhesion assay in vitro. Results The expression of E-cadherin,ICAM-1, CD44 and E-selectin in SW1116 cells were changed significantly following exposure to CO2-insufflation of different pressure groups （ P 〈 0. 05 ）. When 0 h after exposure, with CO2-insufflation pressure ascending, the expression of the E-cadherin, CD44 and ICAM-1 decreased （ P 〈 0. 05 ）. The adhesive cells also decreased gradually with the pressure ascending in adhesion assay （ P 〈 0. 05 ）. Conclusion The expression of adhesion molecules can be affected temporarily and bi-directionaUy by continuous CO2-insufflation. Higher pressure of CO2-insufflation could inhibit the adhesion potential in vitro,which is associated with inhibited expression of adhesion molecules.