纳米纯铁粉对小鼠的毒性和血清生化指标的影响

Study of Toxicity of Nano-iron Particulates and Their Effect on Serum Biochemical Indexes in Mice

目的探讨纳米纯铁粉的急性毒性及其对小鼠血清生化指标的影响。方法按照固定剂量(5 g/kg)法以纳米纯铁粉一次经口灌胃,14天后处死;分别测定肝、肾脏器系数和血清生化指标的变化情况。结果与对照组相比,纳米纯铁粉组小鼠肝脏器系数(0.054 9±0.004 1)明显高于对照组(0.049 3±0.006 2,P<0.05);血清铁(Fe)和总铁结合力(TIBC)则无显著性变化(P>0.05)。纳米纯铁粉组小鼠血清总铁未饱和度(UIBC)、乳酸脱氢酶(LDH)、总胆红素(TBIL)和总胆汁酸(TBA)水平分别为(8.93±7.39)%,(301.78±120.50)U/L,(1.48±0.26)μmol/L和(1.01±0.42)μmol/L,明显低于对照组的(12.65±9.82)%,(407.75±101.29)U/L,(1.96±0.59)μmol/L和(1.73±1.02)μmol/L,差异有显著性(P<0.05)。纳米纯铁粉组小鼠血清丙氨酸氨基转移酶(ALT)、天冬氨酸氨基转移酶(AST)、尿酸(UA)、尿素氮(BUN)、肌酐(Cr)和葡萄糖(GLU)水平与对照组相比有所降低,但差异无显著性(P>0.05)。结论纳米纯铁粉基本无毒,其对血清生化指标的影响可能与一般铁材料有所不同。

Objective To explore the acute toxicity of nano-iron particulates and their effect on serum biochemical indexes in mice. Methods The mice were treated with a single oral dose of nano-iron particulates at 5 g/kg body weight. After 14 days, the mice were sacrificed, the visceral coefficients of liver and kidney were calculated and the serum samples were collected for biochemicel analysis. Results The liver visceral coefficient of nano-iron particulates group （0.054 9 ±0.004 1） was obviously higher than that of control group （0. 049 3±0. 006 2, P〈0.05）. Compared with that of the control group, the serum iron and total iron-binding capacity （TIBC） of nano-iron particulates group have no significant difference （P〉0.05）. The serum unsaturated iron-binding capacity （UIBC）, LDH, TBIL and TBA of nano-iron particulates group were （8.93± 7.39） %, （301.78 ± 120.50） U/L, （ 1. 48 ± 0. 26） μmol/L and （ 1. 01 ± 0.42）μmol/L respectively, significantly lower than those of the control group（ 12.65 ± 9.82） %, （407.75 ± 101.29） U/L, （ 1. 96±0.59） μmol/L and （1.73±1. 02）μmol/L （P〈0.05）. The serum ALT, AST, UA , BUN, Cr and GLU of nano-iron particulates group have the tendency to be lower but with no significant difference compared with those of the control group （P〈0. 05）. Conclusions In general the nano-iron particulates could be considered less toxic and might show some different effect on serum biochemical indexes compared with the ordinary iron material.