慢性渐进分级压迫大鼠脊髓损伤动物模型的研制

THE DEVELOPMENT OF ANIMAL MODELS OF CHRONIC PROGRESSIVE COMPRESSION INJURY OF RAT SPINAL CORD

为设计并验证一种较理想的慢性渐进性压迫脊髓损伤动物模型,采用切除T9椎板,置入慢性渐进压迫装置,多次渐进旋入螺钉,造成大鼠胸段脊髓不同程度的慢性渐进性压迫损伤的方法造模,根据椎管侵占率将实验组分为轻度(<30%)、中度(30%～60%)、重度压迫(>60%)三组,行组织病理学、CBS、MEP和SEP等检查。结果显示,椎管侵占率达12.3%～82.5%;组织病理学检查发现三组动物基本再现了不同程度的慢性压迫性脊髓损伤的特点;CBS、MEP和SEP等检查显示三组动物呈不同程度的脊髓神经功能减退,且行为功能检查和神经电生理检查呈显著正相关。表明该实验动物模型是一种较为理想的、能合理分级的慢性渐进压迫脊髓损伤动物模型。

In order to design and verify a rational animal model of chronic progressive spinal-cord compression injury (CPSCI), CPSCI was caused in Wistear rats by implanting a graded chronic progressive compressive equipment after T9-laminectomy; the experimental groups were divided into three subgroups: the mild (< 30 %), the moderate (30 % to 60 %) and the severe (> 60 %) according to the spinal-canal-occupying rates; and the specimens were examined by CBS, MEP, SEP and histopathology. The results showed that the spinal-canal-occupying rates ranged from 12.3 % to 82.5 %; the histopathological examinations showed varied-degree CPSCI in all three groups; the CBS, MEP and SEP examinations showed varied-degree spinal-nervous hypofunction and the behavior function examination was significantly correlated with the electro-neurophysiological examination, suggesting that the experimental animal model can be regarded as a rational animal model of CPSCI that can be rationally graded.