9种人参皂苷同时测定方法及在人参质量鉴别中的应用

A Method of Simultaneous Determination of 9 Ginsenosides for Identifying the Quality of Ginseng

建立可同时测定人参皂苷Rg1、Re、Rf、Rg2、Rb1、Rc、Rb2、Rb3和Rd含量的反相高效液相色谱(RP-HPLC)方法.采用Agilent Zorbax SB-C18柱,以乙腈-水-0.05%磷酸为流动相,流速1.5 mL/min,柱温35℃,检测波长203 nm.在此色谱条件下,各组分在60 min内均得到较好分离,回收率符合含量测定要求.运用该方法对不同产地人参进行含量测定,道地药材主根中9种人参皂苷总含量为1.19～1.45%,须根为5.47～6.90%,3个非道地药材主根分别为1.03%、1.04%、1.85%.聚类分析结果表明,根据测定的9种皂苷含量能准确区分人参的主根与须根,并判断其道地性.

A reversed phase high performance liquid chromatographic （RP-HPLC） method was developed for simultaneous determination of ginsenosides Rg1, Re, Rf, Rg2, Rb1, Rc, Rb2, Rb3 and Rd. The mobile phase was composed of CH3CN-0.05% H3PO4-H20 and the flow rate was 1.5 mL/min. Agilent Zorbax SB-C18 column was adopted and the column temperature was controlled at 35℃. The wavelength of detector was set at 203 nm. All the nine ginsenosides were separated well within 60 min. The recovery result was acceptable. Ginsenosides in ginseng roots from different geographic region were determined by the method above. For genuine herbs, the total content of nine ginsenosides in main root lies between 1.19% and 1.45%, and in root fiber, 5.47% -6.90%. For herb from region other than genuine, ginsenosides in main root were 1.07% , 1.03% , 1.85% , respectively. The results of cluster analysis and principal component analysis indicated that main root and root fiber could be discriminated by the content of nine ginsenosides assayed, and genuine herbs could be distinguished from non-genuine ones.