三硝基甲苯及其代谢产物与脱氧核糖核酸的结合作用

Deoxyribonucleic acid-binding specificity of trinitrotoluene and its metabolites

运用吸收光谱移动法观察了三硝基甲苯（ＴＮＴ）及其代谢产物与脱氧核糖核酸（ＤＮＡ）的结合作用。结果表明：在ＴＮＴ及其代谢产物浓度一定的条件下，改变ＤＮＡ浓度，结果随着ＤＮＡ浓度的降低，ＴＮＴ及其代谢产物的最大吸收峰λｍａｘ出现短移，且吸收强度也随之增大。当ＤＮＡ浓度固定时，改变ＴＮＴ及其代谢产物浓度时，发现ＴＮＴ（１００μｍｏｌ／Ｌ）、４－Ａ（５０，２５μｍｏｌ／Ｌ）使ＤＮＡλｍａｘ２２８ｎｍ峰消失。提示：ＴＮＴ及其代谢产物与ＤＮＡ有不同程度的结合作用。

The binding specificity of deoxyribonucleic acid(DNA)with trinitrotoluene(TNT) and its metabolites (2-amino-4,6-dinitrotoluene 2-A;4-amino-2,6-dinitrotoluene,4-A;2,4-diamino-6-nitrotoluene,2,4-DA) in vitro was investigated by the shift of absorption spectrum. It was found that after treatment with DNA,the peak of absorption spectum of TNT and its metabolites was shifted toward short wave length with increased absorption intensity. Absorption intensity of DNA decreased after DNA was incubated with different concentrations of TNT and its metabolites. The results indicated that a binding of DNA with TNT or the metabolites of TNT occured.