摘要
心包的功能是保护心脏和减少心脏搏动时的摩擦，是否还有内分泌功能。为此我们实验以ｐＢＲ３２２为血浆心钠素（ＡＮＦ）质粒载体，将７５０ｂｐ的ｒ－ＡＮＦｃＤＮＡ克隆到ＰｓｔＩ位点上，制备ｒ－ＡＮＦｃＤ－ＮＡ，经异硫酸氰胍制备法提取大鼠心房和人心包总ＲＮＡ。应用分子杂交技术，将α－３２Ｐ标记的大鼠心房ｃＤＮＡ与ＲＮＡ进行分子杂交。本实验证明，人类心包存在ＡＮＦ基因转录和表达，心包组织也存在ＡＮＦ。提示人类心包除已知功能外，尚具有重要的肽类激素内分泌功能。
Thefunctionofthepericardiumisbelievedtoprotecttheheartfromfriction.Inthisimmunohistochemicalexperiment,atrialnatriureticfactor(ANF)immunegranuleswerefoundinthepericardium.Aserialstudyofthehumanpericardialfunctionhasbeencariedoutsince1989.ThretypesofANFwereidentifiedinpericardiumwithhighpresureliquidchromatography(HPLC),ra-dioimmunoasay(RIA),immunohistochemistryandimmunoelectromicroscopy,mainlya-hANFandr-hANFwhichweredifuselydistributedincytoplasmofmesothelialcels.InordertoconfirmtheoriginoftheANFinpericardium,totalRNAguanidinethiocyanatepreparation,750bplengtha-rANFcDNAsegmentwasinsertedtothesiteofPstIwithANFatPBR822carrier.Dependingonran-domprimerlabelgsystem,thetotalpericardialRNAwasextractedbyonestepmethodofChom-czynskiafterRNAprobewaspreparedfromDNAclone.TheresultsshowedthatANFgeneconver-sionandexpressionexistedinhumanpericardiumtissue.Usingthedot-blothybridizationandautora-diography,humanpericardiumwasshowntohaveendocrinefunctionofthepeptidewhichisveryim-portant.
出处
《中华心血管病杂志》
CAS
CSCD
北大核心
1996年第1期49-51,共3页
Chinese Journal of Cardiology
关键词
心包
心钠素
基因转录
pericardiumartrialnatriureticfactordot-blothybridization
