高效液相色谱法快速测定人血、尿中的阿托品

Determination of atropine in plasma and urine by HPLC

目的建立血、尿中阿托品的高效液相色谱快速分析方法。方法以正常空白人血浆和尿液添加标准阿托品对血、尿样品的前处理方法、仪器测试条件、线性范围、精密度、回收率进行全面考查,旨在对血、尿中阿托品的浓度进行快速测定。结果该方法在血、尿中的线性范围是0.2～5.0mg·L-1;最低检测浓度为0.2mg·L-1;日内、日间精密度在血液中分别小于3.2%和4.0%;尿液中分别小于3.6%和4.7%;血尿中阿托品回收率分别在(97.0±2.1)%和(97.8±2.9)%以上。结论所建方法便捷、实用,全部分析过程可在40min内完成,适用于临床血药浓度监测和法医学鉴定。

OBJECTIVE For the purpose of more accurate and rapid analysis of atropine in plasma and urine, a reversed-phase high performance liquid chromatography （HPLC） method was developed. METHODS Standard atropine was added to the blank plasma and urine when the samples were pretreated. The pretreatment method of samples, the linear range, the precision, the recoveries in the plasma were investigated by using of human plasma and urine spiked with standard atropine. RESULTS The linear range were 0. 2-5.0mg·L^-1 , r = 0. 999 2 in plasma and r = 0. 998 8 in urine. The detective limits were 0. 2mg·L^-1. The intra and inter-day precision of assay for atropine were less than 3.2% and 4. 0% （n = 5） in plasma,3. 6% and 4. 7% in urine respectively. The recoveries of atropine were more （97. 0 ±2. 1 ） %in plasma and （97. 8± 2.9）% in urine. The HPLC method had been used to investigate the concentration of atropine in many forensic medicine cases. CONCLUSION The HPLC method of quantitative analysis atropine is simple, rapid and accurate. It is suitable for the concentration monitoring of atropine in the clinical first aid and forensic medicine.