PPARγ2表达促毛囊bulge细胞向皮脂腺定向分化的研究

Differentiation of hair follicle bulge cells into sebaceous gland cells induced by peroxisome proliferator activation receptor γ 2

目的探讨毛囊bulge细胞向皮脂腺细胞分化的机制。方法构建携带过氧化物酶体增殖体激活受体γ2(peroxisomeproliferatoractivationreceptorγ2,PPARγ2)基因的绿色荧光蛋白(GFAP)质粒,通过质脂体转染到体外分离培养毛囊bulge细胞中,以转染空质粒的毛囊bulge细胞为对照,观察细胞的分化情况,运用免疫细胞化学检测细胞PPARγ和上皮膜抗原(epithelialmembraneantigen,EMA)的表达,油红O染色观察细胞脂滴合成情况。结果荧光显微镜下观察到已转染质粒的细胞呈现绿色荧光,能稳定表达PPARγ2mRNA,该细胞分化3周左右,部分细胞胞浆内出现脂滴,PPARγ、EMA及油红O染色阳性,而对照组为阴性,细胞内未见脂滴。结论毛囊bulge细胞可以分化为皮脂腺细胞,PPARγ2基因在其中起着重要作用。

Objective To study the mechanism of differentiotion of hair follicle bulge cells into sebaceous gland cells. Methods The peroxisome proliferator activation receptor γ2 （PPARγ2） expression plasmid of pEGFPC1-PPARγ2 gene was constructed, then transfected into the hair follicle bulge cells from 7-day male Wistar rats mediated by liposome, and those transfected with blank pEGFPC1 plasmids were used as controls. The morphological changes of the hair follicle bulge cells were observed, and immunocytochemical staining were used to detect expression of PPAR and EMA in hair follicle bulge cells after transfection, and oil red O staining were used to observe the lipid droplet synthesis of cells. Results The hair follicle bulge cells that were transfeeted the plasmids expressed bright-green fluorescence. The experimental cells could express PPARγ2 gene, and 3 weeks later the lipid droplets were observed in cytoplasm, and the positive expression of PPARγ, EMA and oil red O staining, but those phenomena did not occurred in control cells. Conclusion The hair follicle bulge cells have the capability to differentiate into sebaceous gland cells in vitro, and PPARγ2 play an important role in differentiation.