氯胺酮对SD幼鼠海马神经元细胞凋亡和突触素表达的影响

Effect of ketamine on apoptosis and synaptophysin expression in hippocampal neural cell of SD infant rats

目的观察氯胺酮对7d龄SD幼鼠海马神经元细胞凋亡和突触素(P38)表达的影响。方法7d龄SD幼鼠共30只,随机分成3组,分别注射氯胺酮25mg/kg(K1组)、50mg/kg(K2组),对照组注射生理盐水50ml/kg(K0组),24h后取材,采用Tunel法检测神经元细胞凋亡,免疫组化染色和Westernblot技术检测SD幼鼠海马突触素的表达,进行图像分析。结果注射氯胺酮后24h,海马神经元细胞凋亡计数K0组(5.3±1.7)、K1组(9.5±4.2)、K2组(23.4±7.6),免疫组化染色突触素灰度值:K0组为(174.11±4.68),K1组为(181.36±4.17),K2组为(198.25±3.06)。Westernblot突触素蛋白电泳条带积分光密度值K0组为(4007±758),K1组为(2621±465),K2组为(987±183)。结论注射氯胺酮后24h,SD大鼠海马神经元细胞大量凋亡,突触素表达减少可能和氯胺酮神经毒性有关。

Objective To observe the effect of ketamine on apoptosis and synaptophysin expression in 7- day-old infant SD rat hippocampal neural cells. Methods Thirty SD rats of 7 days old were randomly assigned to receive the injection of 25 mg/kg ketamine （ K1 group） or 50 mg/kg （ K2 group） or 50 ml/kg normal saline （K0 group）. In 24 h after injecton, all rats were killed and the synaptophysin was tested by using immunhisto- chemistry and Western blotting, the apoptosis of neuronal cell by Tunel. Results In 24 h after injection of ketamine ,the number of apoptotic neural cells increased,which of K0 group was （5.3 ± 1.7）, K1 group （9.5 ± 4.2） , K2 group （23.4 ± 7.6） , and the grey values of synaptophysin by immunhistochemistry of K0 group was （174.11 ±4.68）, K1 group （181.36 ±4. 17）, K2 group （198.25 ±3.06）, and the OD values of synaptophysin piece decreased, which of K0 group （4 007 ±758）, K1 group （2 621 ±465）, K2 group （987 ± 183）. Conclusion In 24 h after treatment with ketamine, the expression of synaptophysin was decreased in hippocampl neural cells, the number of apoptotic neuronal cells were increased, which may be involved in neurotoxicity caused by ketamine.