体外培养人胎儿雪旺氏细胞的增殖及其细胞动力学研究

A STUDY ON THE PROLIFERATION AND CELL DYNAMICS OF HUMAN FETAL SCHWANN CELLS IN VITRO

新鲜和液氮保存3个月的人胎儿雪旺氏细胞体外培养8天,细胞数量增加一倍,培养液中加入二丁基环磷酸腺苷和牛脑垂体浸出液,细胞增倍时间缩短近一倍.流式细胞计测定结果显示,培养早期,S期、G2+M期细胞所占比例<5%,随着培养时间的延长,其比例逐渐增加,第6天达高峰,环磷酸腺苷和垂体浸出液作用3天,S期、G2期+M期比例达15%.实验结果表明:人胎儿雪旺氏细胞在体外增殖缓慢,环磷酸腺苷和垂体浸出液能刺激雪旺氏细胞分裂;冷冻复苏后的雪旺氏细胞其生长特征以及对环磷酸腺苷和垂体浸出液的反应与新鲜细胞相同.

The fresh and cryo-preserved human fetal Schwann cells were found to have doubled in number after cultured in DMEM plus 15% calf serum for 8 days, but the proliferate time of cultivation was reduced to 4 days when dibutyryl-cyclic AMP (dbcAMP) and Bovine Pituitary Extract (BPE) were added to the medium. Flow cytometer assay showed that cells of S+G2 + M phases were 5% at 2 DIV, and gradually increased and reached their peak at 6 DIV. When dbcAMP and BPE were added to the medium, cells of S+G2 + M phases were over 15% at 4 DIV. The result indicates that dbcAMP and BPE can stimulate the proliferation of Schwann cells in vitro, and that cryo-preserved Schwann cells are similar to fresh Schwann cells in growth and in response to dbcAMP and BPE.