胃癌COX-2的表达与微血管密度及相关临床病理因素的关系

Relationship of Microvessel Density Pathological Features and Cyclooxygenase-2Expression in Human Gastric Cancer

目的 :探讨胃癌COX - 2的表达与微血管密度及相关临床病理因素的关系。方法 :采用免疫组化S -P法检测 4 6例胃癌的COX - 2表达 ,采用CD34单克隆抗体进行微血管内皮细胞染色 ,计算微血管密度 (MVD)分析与相关临床病理因素的相关性。结果 :COX - 2在胃癌组织表达的阳性率为 71 6 %。COX - 2的高表达与胃癌淋巴结转移、浸润深度、分化程度和临床分期有关 ,与肿瘤大小、腹膜转移、血行转移无显著相关性。COX - 2表达阳性、淋巴转移阳性、浸润程度深者MVD值明显增高。结论 :COX - 2的表达对胃癌细胞的演进和血管形成可能起重要作用 ,是判断预后和指导治疗的有效指标。

Objective： To evaluate the expression of cyclooxygenase - 2 （ COX - 2） in gastric carcinoma and its relationship with microvessel density （MVD） and pathological features. Methods： Immunohistochemical stain was used for detecting the ex- pression of COX - 2 and CD34 in 46 resected specimens of gastric carcinoma. Paracancerous tissues were examined as control. Results： The rate of COX - 2 expression in gastric cancers was significantly increased compared with that in paracancerous tissues （71.6%, 0%, P 〈 0.01 ）. In 39 gastric carcinoma specimens with lymph node invasion, the rate of COX - 2 expression was higher than that in the specimens without invasion （87.2%, 14.3%, P 〈 0.05）. The rate of COX - 2 expression in the speci- mens with depth of invasion T3 and T4 was higher than that in the specimens with depth of invasion T1 and T2 （89.2%, 55.6%, P 〈 0.05）. The rate of COX- 2 expression in cancers of TNM stage Ⅲ and Ⅳ was significantly higher than that in stage Ⅰ and Ⅱ （ 89.3 %, 55.6 %, P 〈 0.05 ）. The rate of COX - 2 expression in cancers of poorly differentiated and undifferentiated adenocarcinoma was higher than that of ,well and moderately differentiated （ 100%, 42.1%, P 〈 0.05）. The MVD values in COX - 2 positive （60.69 ± 28.15） and lymph node invasion positive （59.31 ± 18.6） groups was significantly higher than those in COX - 2 negative and lymph node invasion negative groups （41.05 ± 17.96, 41.65 ＋_ 14.45, respectivel, P 〈 0.05}, and in depth of invasion T3 and T4 was significantly higher than in depth of invasion T1 and T2 as well （58.60± 18.24, 43.54± 15.05, respectively, P 〈 0.05）. Conclusion： These data indicated that overexpression of COX- 2 in gastric carcinoma was well related to its lymph node metastasis, and clinical stage, and differentiation degree and depth of the invasion, but it did not correlate with the size of tumor , blood- borne metastasis, and peritoneal dissemination （ P 〉 0.05）. The expression of COX- 2 might play a im- portant role in neovascularization and tumor progression. It may serve as a prognosis factor and the guide for clinical decisions.