大鼠腹主动脉移植术后血管内皮细胞SDF-1的表达与内膜增厚程度相关性的研究

Study of the Correlation between Neointima Thicking Rate and SDF-1 Expression onthe Endothelial Cells of Rats Abdominal Aorta Grafts

目的 :探讨基质衍生因子 - 1(SDF - 1)在移植后大鼠腹主动脉内皮细胞上的表达量与内膜增生程度之间的相关性。方法 :建立雄性Wistar大鼠至雄性SD大鼠的腹主动脉原位移植模型 ,测量移植后大鼠腹主动脉新生内膜的厚度 ,免疫组织化学检测同时间点移植物内皮细胞上SDF - 1的表达。结果 :发现大鼠腹主动脉内皮细胞上SDF - 1的表达量与内膜增生的程度相关性高。结论 :SDF - 1在大鼠腹主动脉移植术后介导造血干细胞参与损伤组织的修复 ,并可预警移植物血管硬化以及随后的慢性移植物失功。

Objective： To investigate the correlation between Stromal cell derived factor- 1 （SDF- 1 ） expression and neointima thicking rate of rat abdominal aorta grafts at the same time - point post - transplantation. Methods ： Male Sprague - Darley rats received abdominal aorta grafts from male Wistar abdominal arteries. Neointima thickness of rat abdominal aorta gratis was measured and immunohischemistry was conducted for checking the expression of SDF- 1 on the endothelial cells. Results： The correlation between SDF- 1 expression and neointima thicking rate at the sanae time- point post- transplantation was significant. Conclusion： SDF- 1 participated in the repair process of rat abdominal aorta grafts by mobilizing hematopoietic stem cells to injured parts. Furthermore, SDF- 1 is predictive of the subsequent development of arteriosclerosis and chronic graft dysfunction （CGD）.