针刺不同腧穴对抑郁症模型大鼠行为学的影响

Effects of acupuncture at different acupoints on behaviors in depression model rats

目的:观察针刺不同腧穴治疗抑郁症的疗效差异。方法:选择OpenField法评分相近的70只成年SD雄性大鼠随机分为正常对照组、模型组、空白对照组、药物组(氟西汀组)、针刺1组(“百会”“神庭”组)、针刺2组(“内关”“三阴交”组)和针刺3组(“百会”“神庭”“内关”“三阴交”组),每组各10只。应用分养和长期不可预见性中等强度应激造成抑郁症模型大鼠。以OpenField法和糖水消耗试验对照研究慢性应激诱导的抑郁症模型大鼠行为学的改变。结果:与正常对照组比较,模型大鼠行为学评分降低,糖水消耗量减少。与空白对照组比较,各治疗组大鼠在治疗过程中行为学评分升高,糖水消耗量增加;在治疗21天后,各针刺组之间的行为学评分增加差异无显著性意义(P>0.05);但在治疗14天时,针刺1组糖水消耗增加和针刺3组的垂直运动评分增多,差异有显著性和非常显著性意义(P<0.05,P<0.01)。结论:针刺治疗可以改变分养和长期不可预见性中等强度应激诱导的抑郁症模型大鼠的行为学异常;“百会”“神庭”穴组或与“内关”“三阴交”穴合用,在治疗抑郁症方面有一定优势。

Objective To observe therapeutic effects of acupuncture at different acupoints on depression. Methods Seventy adult male rats with similar Open-Field score were randomly divided into a normal control group, a model group, a blank control group, a drug treatment group, an acupuncture group Ⅰ [“Baihui” （GV 20）, “Shenting” （GV 24）], Ⅱ [“Neiguan” （PC 6）, “Sanyinjiao” （SP 6）], and Ⅲ （“Baihui”, “Shenting”, “Neiguan” and “Sanyinjiao”）, 10 rats in each group. Separation feeding, long term unpredictability and medium stimulation stress techniques were applied to develop depression model rats. Changes of behaviors were investigated with Open-Field method and sucrose consumption trial. Results Compared with the normal control group, the score for the behavior and the consumption of sucrose reduced in the depression model group; compared with the blank control group, the score for the behavior and the consumption of sucrose increased in all the treatment groups during treatment. There was no significant difference among the acupuncture groups in the increase of behavior scores 21 clays after treatment （P〈0. 05）, and there were significant differences in the increase of consumption of sucrose in the acupuncture group Ⅰ and the increase of score for vertical movement in the acupuncture group Ⅲ 14 clays after treatment （P〈 0.05, P〈0.01 ）. Conclusion Acupuncture can change the behavior abnormality induced by separation feeding, long term unpredictability and medium degree stimulation depression model rats and different acupoint selection is a factor in aeupunture treatment.