艾灸“肺俞”“膏肓”影响BLM_(A5)诱导肺纤维化大鼠TGF-β1表达实验研究

Effects of moxibustion at Feishu (BL 13) and Gaohuang (BL 43) on expression of TGF-β1 in the bleomycin -induced pulmonary fibrosis

目的:探索针灸阻抑肺纤维化的效应机制,为针灸介入肺纤维化防治提供实验依据。方法:将SD大鼠140只随机分为4组:空白组、模型组、艾灸组、泼尼松组,每组35只。气管内注入博莱霉素制作大鼠肺纤维化模型,造模后7d开始治疗,以5mg艾绒灸其双侧“肺俞”“膏肓”,每穴3壮,每天1次,10d为一疗程,共治疗3个疗程后处死动物,采用PCR检测其转化生长因子β1(TGFβ1)mRNA表达。结果:肺组织TGFβ1mRNA表达分别为:空白组1.0258±0.0057,模型组2.8104±0.2905,艾灸组1.6174±0.1136,泼尼松组1.7176±0.1079。艾灸组、泼尼松组与模型组比较,P<0.01,艾灸组与泼尼松组比较,P>0.05。结论:艾灸“肺俞”“膏肓”与泼尼松治疗均能显著抑制肺纤维化大鼠肺组织TGFβ1mRNA表达。

Objective To explore the mechanism of moxibustion arresting the pulmonary fibrosis and provide experimental basis for prevention and treatment of pulmonary fibrosis with acupuncture and moxibustion. Methods One hundred and forty SD rats were randomly assigned to 4 groups： a blank group, a model group, a moxibustion group and a prednisone group, 35 rats in each group. The 3 groups expect the blank group were injected with bleomycin via trachea to induce experimental pulmonary fibrosis model, and 7 days after modeling, they were treated with moxibustion at bilateral Feishu （BL 13） and Gaohuang （BL 43）, 3 cones each point, once each day, 10 days constituting one therapeutic course with an interval of one day between courses. After 3 courses, all rats were killed and expressions of TGF-β1mRNA were detected with PCR method. Results The content of TGF-β1mRNA in the pulmonary tissue in the moxibustion group and the prednisone group was significantly lower than the model group （P〈0.01）, and there was no significant difference between the moxibustion group and the prednisone group （P〉 0.05）. Conclusion Both moxibustion at Feishu （BL 13） and Gaohuang （BL 43）, and prednisone treatment can significantly suppress the expression of TGF-β1 mRNA in the pulmonary tissue in the rat of bleomycin-induced pulmonary fibrosis.