电针对局灶性脑缺血大鼠神经功能缺损及病理形态的影响

Effects of electroacupuncture on neurological deficit and pathomorphologic lesion in the rat of focal cerebral ischemia

目的探讨电针对脑梗死的作用规律。方法采用热凝闭法建立SD大鼠MCAO模型,电针“百会”“大椎”穴,采用NSS评价神经功能缺损情况、TTC染色检测脑梗死体积、HE染色观测脑组织病理改变。结果MCAO可造成大鼠神经功能缺损和脑梗死及脑组织病理损害,但上述损害可随着缺血时间的延长而有不同程度的自愈趋势;电针可使不同缺血时相的各种损害减轻。结论电针具有改善神经功能缺损、缩小脑梗死体积和减轻缺血性病理损害程度的作用;研究还提示针灸治疗缺血性脑卒中进行早期干预具有十分重要的临床意义。

Objective To probe into regularity of effects of electroacupuncture on focal cerebral infarction. Methods Focal cerebral ischemia model was established by middle cerebral artery heat-occlusion （MACO） method. Electroacupuncture was givent at Dazhui （GV 14） and Baihui （GV 20）. NSS was used for evaluation of neurological impairment, TTC staining method for detection of the infarct volume, and HE staining method for investigation of the pathomorphologic lesion of the brain. Results MCAO could induce neurologic impairment, cerebral infarction and cerebral pathological lesion in the rat, all of these changes could be cured spontaneously in varying degrees with prolongation of ischemic time, but electroacupuncture could relieve the lesions to a certain extents. Conclusion Electroacupuncture can improve neurologic deficit impairment, reduce the volume of cerebral infarction and ischemic pathological lesion, early interfere of acupuncture and moxibustion is of very important clinical significance for treatment of ischemic apoplexy.