经皮穴位电刺激足三里对抗大鼠运动性疲劳

Effect of transcutaneous electric acupoint stimulation at Zusanli on exercise-induced fatigue in rats

目的:观察经皮穴位电刺激足三里对大鼠抗运动性疲劳能力的影响。方法:实验于2005-01在浙江中医学院实验动物研究中心完成。选用6周龄健康SD大鼠40只,雌雄各半。采用大鼠游泳力竭运动疲劳模型。随机将大鼠分成边游泳边治疗组(经皮穴位电刺激治疗10次,即每日力竭游泳后予经皮穴位电刺激治疗,1次/d,共10次),造模后即时治疗组(即第10天游泳后予经皮穴位电刺激治疗1次),游泳后治疗组(第11天起每日予经皮穴位电刺激治疗1次,共10次),造模组(不给予治疗)4组,每组10只。于实验前、实验第10天和第20天观察经皮穴位电刺激对大鼠游泳耐力时间、心率、血糖、血乳酸含量、红细胞超氧化物歧化酶活性及体质量的影响。体质量在大鼠早晨未进食前测试。血糖测定采用葡萄糖氧化酶法。血清乳酸测定采用对羟基联二苯法。血清超氧化物歧化酶活力测定采用黄嘌呤氧化酶法。计量资料用t检验,多组间用F检验,计数资料用χ2检验。均以P<0.05为差异显著的标准。结果:在整个实验过程中造模后即时治疗组及造模组各有1只大鼠在游泳训练中淹死,其余38只均进入结果分析。①大鼠力竭游泳时间:边游泳边治疗组治疗后比造模前有明显延长眼(19.42±2.26),(15.50±1.03)min熏P<0.01演;造模组治疗前后保持不变眼(15.35±1.11),(15.00±1.12)min,P<0.01演;边游泳边治疗组明显长于造模组。雌、雄大鼠增长差异不明显。②心率:造模组和造模后即时治疗组大鼠在第10天比造模前明显升高,其中造模后即时治疗组升高幅度低于模型组,而边游泳边治疗组无明显变化;在第20天时模型组没有显著变化,而游泳后治疗组显著降低,在第20天游泳后治疗组低于模型组。③血清血糖浓度:造模组和造模后即时治疗组大鼠在第10天比造模前明显降低,而边游泳边治疗组无明显变化。边游泳边治疗组第10天降低幅度低于模型组和造模后即时治疗组,造模后即时治疗组降低幅度低于模型组;第20天游泳后治疗组和模型组比第10天显著升高。④血清乳酸:边游泳边治疗组和模型组大鼠在第10天含量比造模前明显升高,而造模后即时治疗组无明显变化;第10天边游泳边治疗组和模型组升高幅度高于造模后即时治疗组,其中边游泳边治疗组升高幅度低于模型组;第20天游泳后治疗组和模型组比第10天显著降低。⑤超氧化物歧化酶活性:边游泳边治疗组、造模后即时治疗组和模型组大鼠第10天的均比造模前显著升高;游泳后治疗组和模型组第20天比第10天明显降低。⑥体质量:边游泳边治疗组、造模后即时治疗组和模型组大鼠在第10天均比造模前明显升高;第10天边游泳边治疗组增长幅度明显慢于造模后即时治疗组和模型组;游泳后治疗组和模型组第20天增长明显快于第10天。结论:①经皮穴位电刺激足三里具有延长大鼠力竭游泳耐力时间,延缓过度疲劳的发生,在运动性疲劳方面能够起到一定程度的预防和治疗作用。②经皮穴位电刺激可能通过改善运动性疲劳大鼠整体健康状况,提高能量代谢效率,纠正自由基代谢失衡,减少代谢产物的堆积,从而达到改善运动性疲劳的作用。③经皮穴位电刺激治疗具有明显的即时效应,以及累加效应,可作为对抗运动性疲劳的一种新方法。

AIM： To observe the effects of transcutaneous electric acupoint stimulation at Zusarli in rats on exercise-induced fatigue. METHODS： In January 2005, the experimental study was done in the Experimental Animal Center, Zhejiang College of Traditional Chinese Medicine. Rat model of exercise-induced fatigue was established by the exhaustive swimming in mrs, Forty SD rats, six months, half in each sex, were randomly divided into swimming and treatment group（Stimulation was given 10 times respectively after daily swimming, once a day）, swimming and instant treatment group （once stimulation at the tenth swimming）, post-swimming treatment group （Stimulation was given 10 times,once a day from the eleventh day） and model group, with 10 rats in each group. The swimming endurance, heart rate, and body mass before eating were tested before experiment, and at the 10^th and 20^th days after treatment; glucose （Glu） and lactic acid （LA） in blood were measured with glucose oxidase method and dihydroxydiphenyl method,respectively;and superoxide dismutase （SOD） levels were detected with xanthine oxidase Method. t test was used for analysis of measurement data. Among muhi-groups, F test was adopted. Enumeration data was analyzed with chi-square test. The standard of significant difference was P〈0.05. RESULTS： In the course of experimental study, one rat in the swimming and instant treatment group and model group drowned respectively during swimming exercise, and the others came into the results analysis. ①The swimming time in the swimming and treatment group was significantly prolonged as compared with before experiment [（19.42±2.26 ）vs. （15.50±1.03 ）minutes, P〈0.01]; And the model group had no changes [（15.35±1.11）vs. （15.00±1.12）minutes, P〈 0.01]. There were no differences between the male and female rats） On day 10, the heart rates in the model group and swimming and instant treatment group were promoted significantly as compared with before modeling,and the heart rate in the swimming and instant treatment group was lower than that in the model group, and however, there were no changes in the swimming and treatment group; On day 20, the model group had no changes, and the heart rate was lowered most significantly in the post-swimming treatment group as compared with the model group. ③On day 10, Glu content in the model group and the swimming and instant group was decreased significantly as compared with before,and the swimming and treatment group had no changes. On day 10, Glu content was decreased significantly in the swimming and treatment group as compared with the model group and instant group, and it was lower in the model group than the instant group. On day 20, the Glu content in the post-swimming treatment group and model group was improved significantly than that on day 10. ④On day 10, LA content in the swimming and treatment group and model group was higher than before,and there was no changes in the instant treatment group. The LA content in the former two groups was higher than that in the latter one group,while it was lower in the swimming and treatment group as compared with the model group.On day 20,the LA content in the post-swimming treatment group and model group was lower than that on day 10. ⑤On day 10, SOD activity in the swimming and treatment group, the instant treatment group and the model group was increased significantly as compared with before. On day 20, the SOD activity in the post-swimming group and model group was decreased significantly as compared with thaton day 10. ⑥On day 10, body mass in the swimming and treatment group, the instant treatment group and model group was increased as compared with before, and that in the swimming and treatment group was lower than that in the instant group and model group. On day 20, body mass in the post-swimming group and model group was increased significantly as compared with that on day 10. CONCLUSION： To some extent, transcutaneous electrical stimulation at Zusanli on anti-exercise fatigue has a good effect on prevention and cure,and prolongation of the swimming time by promoting endurance.The therapeutic result may be related with improving the health condition of the arts and the efficiency of body energy metabolism, adjusting unbalance of the free radical metabolism,and reducing the metabolism. It is also found that transcutaneous electric acupoint stimulation has an instant and accumulative effect on exercise-induced fatigue. So it can be applied in the clinical treatment of exercise-induced fatigue.