喉癌中原癌基因c-met蛋白的表达

Expression of proto - oncogene protein c - met in laryngeal carcinoma

目的:研究原癌基因c-met蛋白在人类喉癌组织中的表达,探讨c-met的蛋白表达与喉癌的临床病理特征之间的关系。方法:应用免疫组化SABC法检测80例人类喉癌组织标本及60例声带小结、息肉中c-met的蛋白表达,并行统计学分析。结果:原癌基因c-met蛋白在声带小结、息肉中的表达率为10％,在喉癌组织中的表达率为65％;c-met蛋白的表达与喉癌的临床分期、病理学分级、淋巴结转移均显著相关(P<0．01)。结论:原癌基因c-met蛋白在喉癌组织中高表达且与喉癌淋巴结转移、病理学分期、组织分级密切相关。

Objective： To study the expression of proto - oncogene c - met in human laryngeal carcinomas, and to explore the association between expression of c - met protein and clinical pathological differentiation, stages and lymph node status in laryngeal carcinomas. Methods： Using streptavidin biotinimmuno - histochemistry（SABC）, 80 laryngeal squamous- cell carcinoma specimens and 60 vocal cord polyps with polyclonal antibodies of c - met were studied. Disease stages were determined using the pTNM classification according to UICC criteria （1992）. All specimens were pathologically examined after operation. All data were analyzed by Excel statistic software. Results： Proto - oncogene c - met was expressed highly in laryngeal squamous cell carcinomas （65 %, 52/80）, but lowly in vocal cord polyps （ 10%, 6/ 60）. The difference was statistically significant （P 〈 0.01 ）. C - met was expressed in poorly , moderately, and highly differentiated squamous cell carcinomas at frequencies of 15%, 44%, and 92% respectively. The differences were significant statistically （P 〈 0.01 ）. There were associations between the clinical stages, lymph node status of laryngeal squamous cell carcinomas and c - met （ P 〈 0.01 ）. Conclusion： Proto - oncogene c - met expresses highly in laryngeal squamous cell carcinomas, which is related to pathological differentiation, stage and lymph node status of laryngeal carcinomas.