摘要
为探讨血浆中5-羟色胺和儿茶酚胺的测定方法,采用改良的Keating方法即高效液相色谱电化学检测技术测定兔血浆中5-羟色胺和儿茶酚胺,其方法灵敏、简单,以YWG-C18H37为固定相,用反相色谱测定5-羟色胺,回收率达90%以上,变异系数为3.3%。用反相离子对色谱分析儿茶酚胺,样本经氧化铝吸附后,用0.05mol/LH3PO4洗脱效果更佳。儿茶酚胺回收率分别为79.9%(去甲肾上腺素),95.5%(肾上腺素)和85.5%(多巴胺)。
The concentrations of serotonin and catecholamine in plasma of rabbits were measured using a improved Keating's method:high-performance liquid chromatography with electrochemical detection technique. This method is more specific,sensitive and simple than other methods.The concentration of serotonin was measured by reversed-phase high-performance liquid chromatographic method,in which YWG ODS was used as solid-phase column Φ4×250mm(10μm).The recovery of the serotonin was 93% and the coefficient of variation was 3.3%. The concentration of catecholamine was measured using a revered-phase ion-pair high-performance liquid chromatography technique.It is better to be adsorbed by aluminium oxide than washed by 0.05mol/L H 3PO 4. The recovery of catecholamine was 79.9%(NE) 95.5%(E) and 85.5%(DA)respectively.
出处
《航天医学与医学工程》
CAS
CSCD
1996年第3期217-221,共5页
Space Medicine & Medical Engineering
关键词
高效液相色谱
血清素
儿茶酚胺
血浆
测量
电化学检测
high-performance liquid chromatography serotonin catecholamine plasma measurement electrochemical detection