基质金属蛋白酶抑制剂RECK基因在前列腺细胞株中的表达及意义

Expression and Significance of the Matrix Metalloprofeinase Inhibitor RECK Gene in Prostate Cell Strains

目的:探讨RECK基因及基质金属蛋白酶-9(MMP-9)mRNA在不同前列腺细胞株BPH-1、DU-145、LNCaP及PC-3中的表达差异及其意义。方法:应用RT-PCR检测4种不同前列腺细胞株(BPH-1、DU-145、LNCaP及PC-3)中RECK及MMP-9 mRNA的表达;应用W estern印迹法检测不同前列腺细胞株中RECK蛋白的表达。结果:前列腺癌细胞株DU-145、LNCaP及PC-3中RECKmRNA的表达较良性前列腺增生细胞株BPH-1中明显降低(P<0.01),MMP-9 mRNA表达则明显升高。DU-145、LNCaP及PC-3细胞株中RECK蛋白的表达较BPH-1的表达明显降低。结论:RECK基因在前列腺癌中的表达量明显下降,而MMP-9的表达量明显升高,RECK基因可能是一种抑癌基因,其抑癌作用可能与其抑制MMP-9的表达有关。

Objective： To investigate the expression and significance of RECK gene and MMP-9 in prostate cell strains such as BPH- 1, DU-45, LNCaP and PC-3. Methods ： The expression of mRNA of RECK and MMP-9 was measured by RT-PCR and RECK protein was evaluated by Western blot. Results： The mRNA level of the RECK gene in the prostate carcinoma cell strains, such as DU-45, LNCaP and PC-3, was lower than that in the benign prostate hyperplasia cell lioe BPH-1, while MMP-9 had a higher expression. The protein level of RECK in DU-45, LNCaP and PC-3 was lower than that in the BPH-1. Conclusion ： The RECK gene is supposed to be a kind of tumor suppressor gene, which may act by inhibiting the activity of MMP-9.