“冻干江浙蛇毒”的致突变作用研究

The Mutagenicity of"the Lyophilized Agkistrodon Haly(pallas)"

目的检测“冻干江浙蛇毒”的致突变性,以提供有关致突变的遗传毒性安全评价数据。方法采用小数骨髓微核试验、微生物回复突变试验(Ames)、仓鼠肺成纤维细胞染色体畸变试验(CHL),研究“冻干江浙蛇毒”的致突变作用。结果小鼠骨髓微核试验表明,“冻干江浙蛇毒”三个剂量组(1.0、0.5、0.25mg/kg腹腔注射)的微核出现率与阴性对照组(0.9%NaCl)比较无显著性(P>0.05),与阳性对照组比较有显著性(P<0.01);Ames试验表明,“冻干江浙蛇毒”采用5000、2500、1250、625和312.5ug/皿剂量时,在加和不加S9条件下,对TA97、TA98、TA100和TA102菌株回复突变菌落数结果为阴性;CHL试验也表明,“冻干江浙蛇毒”4.0、2.0、1.0和0.5ug/ml剂量对中国仓鼠肺成纤维细胞(CHL)体外培养染色体无畸变作用。结论“冻干江浙蛇毒”无致突变性,有良好的应用前景。

Objective. To investigate the mutagenieity of＂the Lyophilized Agkistrodon Haly（pallas）＂. Methods：The mutagenieity of＂the Lyophilized Agkistrodon Haly（pallas）＂was studied by the micronueleus test, the Ames test and the CHL test. Results.No signifieant differenees in the mieronuelear frequenees were found in any of the testing groups eompared with negative eontrol in miee （P〉0.05）;The mutagenie aetivity of＂the Lyophilized Agkistrodon Haly（pallas）＂was not observed in TA97, TA98, TA100 or TA102 with or without S9 aetivation under the doses used; In CHL test, the effeet of ehromosomal damage of＂the Lyophilized Agkistrodon Haly（pallas）＂was not found either in CHL cells. Conclusion： ＂The Lyophilized Agkistrodon Haly （pallas）＂ had no mutagenieity,and it might be useful in the future.