WNT4基因在房间隔缺损患者心肌组织中的表达

Expression of WNT4 in Human Myocardium of Atrial Septal Defect

目的:探讨房间隔缺损(ASD)患者心房肌组织中WNT4基因的表达变化,分析WNT4基因与ASD病理生理学之间的关系。方法:获取20例单纯Ⅱ孔型ASD患者和9例正常对照右房心耳肌组织标本,采用RT-PCR技术检测心房肌组织中WNT4基因mRNA的表达丰度。结果:WNT4基因表达于正常和ASD患者心房肌组织中,正常心肌组织中的表达丰度为(54.67±25.85)%,ASD患者心肌组织中的表达丰度为(33.21±20.37)%,ASD患者心肌组织中WNT4基因的表达丰度显著低于正常对照(t=2.4151,P<0.05)。结论:WNT4基因在心肌组织中的异常表达可能与ASD的病理生理学有关,其在ASD患者心肌组织中表达下调可能与ASD病理状态下右房压力负荷增加导致胎儿心脏基因程序启动、心脏特异性染色体重构机制有关。

Objective： To explore the changes of WNT4 gene （Wingless-type MMTV Integration Site Family,Member 4 gene） mRNA expression in human auricular myocardium of secondary atrial septal defect （ASD） and to analyze the relationship between WNT4 gene and the pathophysiology of secondary atrial septal defect. Methods： The total RNAs were extracted from 20 cases of ASD auricular myocardium and 9 cases of normal auricular myocardium. The levels of WNT4 gene mRNA expression in the auricular myocardium of ASD and the normal auricular myocardium were detected using the RT-PCR （Reverse Transcription-Polymerase Chain Reaction） . Results： The WNT4 gene was expressed in both the normal and ASD auricular myocardium. The levels of WNT4 gene mRNA expression were （54.67±25.85）% in the normal auricular myocardium and （33.21± 20.37）% in the ASD auricular myocardium. The levels of WNT4 gene mRNA expression in the ASD auricular myocardium were significantly lower than those in the normal auricular myocardium （t = 2.4151, P 〈 0.05）. Conclusion： The abnormal expression of WNT4 gene in the auricular myocardium may be contributed to the pathophysiology of ASD. The down-regulated expression of WNT4 gene in the auricular myocardium of ASD maybe have relationships with the initiation of fetal cardiac gene program and the cardiac-specific chromatin remodeling caused by the overload of right atrium.