单核细胞趋化蛋白-1和调节活化正常T细胞表达分泌因子与实验性变态反应性神经炎的关系

Relationship between chemokins MCP-1,RANTES and experimental allergic neuritis

目的研究趋化因子单核细胞趋化蛋白-1(MCP-1)和调节活化正常T细胞表达分泌因子(RANTES)与实验性变态反应性神经炎(EAN)发病的关系,探讨EAN的免疫发病机制.方法给Wistar大鼠足垫皮下注射兔坐骨神经匀浆建立EAN模型,用免疫组化技术检测EAN大鼠发病不同时间坐骨神经MCP-1和RANTES的表达.结果EAN组的MCP-1表达第9 d达高峰,随后逐渐下降,第15 d、21 d、28 dMCP-1的表达与前一时间点比较差异均有显著性(均P＜0.01);第9 d、15 d、21 d MCP-1表达均显著高于对照组(均P＜0.001).EAN组第9 d、15 d、21d RANTES表达均显著高于对照组(P＜0.01～0.001),第15 d表达最高.结论MCP-1和RANTES在EAN的发病过程中发挥了重要作用,MCP-1可能起始动作用,RANTES可能与EAN的病情进展有关.

Objective To study the relationship between chemokines monocyte chemoattractant protein-1 （ MCP-1 ） , reduced upon activation of normal T cell expressed and secreted chemokine （RANTES） and experimental allergic neuritis （EAN）. Methods EAN models were induced in Wistar rats by immunization with rabbit sciatic nerves homogenate and complete Freund＇s adjuvant （CFA）. Expressions of MCP-1 , RANTES in the sciatic nerves of experimental rats were detected hy immunohistochemistry technology at different time. Results The maximum MCP-1 positive cells in the sciatic nerves were detected at 9th d. The expression of MCP-1 in EAN group was obviously higher than that in control group at 9th, 15th and 21th d （ all P 〈0. 001 ）. The expression of RANTES in EAN group was also much higher than that in control group at 9th, 15th and 21 th d （P 〈 0.01 -0. 001 ） , and peak expression was at 15th d. Conclusion MCP-1 and RANTES play important roles in the course of EAN.