藻酸双酯钠对大鼠脑缺血再灌注后神经细胞保护作用的研究

Neuroprotective role of PSS following cerebral ischemic reperfusion in rats

目的探讨藻酸双酯钠(PSS)对脑缺血再灌注后神经细胞的保护作用.方法采用线栓法制作大脑中动脉阻断(MCAO)再灌注大鼠模型.PSS治疗组大鼠于再灌注即刻及再灌注24 h、48 h分别给予PSS(18.75 mg/kg)腹腔注射;对照组、假手术组和正常组均同时给予等量生理盐水腹腔注射.观察大鼠神经功能、脑梗死体积、组织学、超微结构和凋亡细胞数的改变.结果(1)PSS治疗组和对照组神经功能评分分别为(1.83±0.75)分和(2.83±0.75)分,脑梗死体积分别为(107.9±12.1)mm3和(150.3±30.5)mm3,两组比较差异有显著性(均P＜0.05);(2)PSS治疗组大鼠脑组织缺血损伤改变明显轻于对照组,神经细胞的组织学和超微结构接近正常;(3)PSS治疗组细胞凋亡数于再灌注后1 h和3 h与对照组相比,差异均无显著性(均P＞0.05),再灌注6 h、12 h、24h、48 h及72 h凋亡细胞数明显少于对照组(P＜0.05～0.01).结论PSS能明显减轻大鼠脑缺血再灌注后的神经功能障碍和神经细胞损害,缩小脑梗死体积,抑制神经细胞凋亡,从而对神经细胞具有保护作用.

Objective To explore the neuroprotective role of propylene glycol alginate sodium sulfate （PSS） following cerebral ischemic reperfusion in rats. Methods Middle cerebral artery occlusion （MCAO） and reperfusion models were made using an intraluminal filament method. The rats in PSS treatment group were injected intraperitoneally with PSS （ 18.75 mg/kg） at 0, 24 and 48 h after reperfusion, same quantity of normal saline was used in control group, sham-operation group and normal group. We studied neurological scores, infarct size, histological and uhrastructure changes of neurons and cell apoptosis. Results （ 1 ） The neurological score was 1.83±0. 75 in treatment group and 2. 83 ±0.75 in control group, the infarct size was （ 107.9 ± 12. 1 ） mm3 in treatment group and （150.3 ± 30.5 ）mm3 in control group. The differences were significant between the two groups （all P 〈 0. 05 ）. （2） The change of ischemic impairment in PSS treatment group was lighter than that of control group. Histological and uhrastructure aheration of neurons in PSS treatment group was nearly to normal. （3） Compared with control group, apoptotic positive cells of PSS treatment group did not change 1 and 3h after reperfusion （ all P 〉0. 05 ）, and obviously decreased 6, 12, 24, 48 and 72 h after reperfusion （ P 〈0. 05 - 0. 01 ）. Conclusion PSS may obviously improve neurological impairment, reduce infarct size and alleviate apoptosis after ischemic reperfusion. It plays protective effect on neurons.