玉米骨干自交系品质基因SSR标记遗传多样性研究

Studies on Genetic Diversity of Quality Gene in Inbred Lines of Maize Based on SSR Markers

选用玉米品质基因第2条染色体上的2对和第7条染色体上的3对SSR引物,对32个玉米自交系材料进行遗传多样性分析。结果表明:不同引物在不同自交系间多样性范围与程度都不相同,umc1066具有最好的多态性,带型稳定,重复性好。在o2基因内的SSR标记区域变异具有独立性,并非保守序列。具有o2基因的自交系与不具有o2基因的自交系,在基因的等位区段具有明显差异;在都具有o2基因的自交系间o2基因区段内的SSR标记具有多样性;具有o2基因的材料与优良自交系之间具有较好的多态性。

A set of 2 maize SSR primers located on the 2th maize chromosomes and a set of 3 maize SSR primers located on the 7th maize chromosomes were used to investigate the genetic diversity of 32 inbred lines of maize. The result showed that all SSR primers were distinct in the range and degree of diversity in different inbred lines. Umc1066 had the best diversity, The SSR markers areas in o2 gene had independent character in variation and they weren＇t conservative sequence. There was a good diversity in between the materials with o2 gene and good inbred lines. Which layed the good foundation for setting up the SSR marker - assisted breeding system of QPM. SSR markers play an important role in variety identification of inbred lines of maize and study of genetic diversity.