鼻咽癌患者EBV感染与细胞免疫水平关系研究

Epstein-Barr Virus Infection and Its Correlation to Changes of Cell Immunity in Nasopharyngeal Carcinoma

目的探讨鼻咽癌患者EBV感染与细胞免疫水平变化的相关性。方法采用PCR技术和流式细胞术(FCM),检测55例鼻咽癌患者EBV感染状况和细胞免疫水平,分析二者的相关性,并与40例健康体检者对比分析。结果鼻咽癌患者外周血CD3+细胞、CD4+细胞明显减少,CD4+/CD8+比值明显下降,NK、CIK细胞占外周血淋巴细胞总数的百分比明显增高,与正常组比较差异有显著性(P<0.01);EBV阳性患者与阴性患者比较,CD3+细胞、CD4+细胞、CD4+/CD8+比值有明显差异(P<0.01),而NK细胞、CIK细胞占外周血淋巴细胞总数的百分比无明显差异(P>0.05)。结论EBV感染进一步抑制NPC患者细胞免疫功能,使病情进一步发展。监测EBV感染状况和细胞免疫水平,对鼻咽癌早期诊断、病情进展判断具有积极的临床意义。

Objective To explore the correlation of EBV infection to changes of cell immunity in Nasopharyngeal Carcinoma （NPC）. Methods EBV infection of 55 patients with NPC was detected by PCR. T lymphocyte, NK and CIK cell activities were measured by flow cytometry. 40 normal healthy volunteers served as control. Results The mean value of T lymphocyte cell of NPC patients was significantly lower than that of control group （P〈0. 01 ） ; Infection of EBV was significantly correlated to T lymphocyte cell activities in NPC （P〈0. 01 ）, but not correlated to CIK and NK cell activities （P〉0. 05）. Conclusion EBV may inhibit cell immunity and participated in development of NPC. Detecting both EBV and changes of cell immunity can be potentially useful in early diagnosis and selection of a poor prognostic subgroup of NPC patients.