摘要
目的观察125I偶联VEGF反义寡核苷酸(125I-ASODN)对膀胱癌细胞VEGF表达的抑制情况。方法采用免疫组化法观察125I-ASODN对膀胱癌细胞VEGF表达的影响,并观察条件培养液对内皮细胞生长的抑制作用。结果免疫组化结果显示对照组VEGF阳性细胞数为平均89.1/100个细胞,125I-Na处理组为90.3/100个细胞,ASODN组为10.3/100个细胞,125I-ASODN组为4.3/100个细胞,125I-ASODN组和ASODN组与对照组及125I-Na组比较,差异有显著性意义(P<0.05);对照组、125I-Na处理组、ASODN组、125I-ASODN组D值分别为0.398±0.031,0.387±0.010,0.298±0.013和0.148±0.011,125I-ASODN组和ASODN组D值与对照组及125I-Na组比较,差异有非常显著性(P<0.01)。结论125I-ASODN能够明显抑制膀胱癌细胞的VEGF表达,可作为临床抗血管生成治疗膀胱癌的一种有效方法。
Objective To study the inhibition effect of VEGF expression in human bladder carcinoma cells by ^125I-coupled antisense oligonucleotides (^125I-ASODN). Methods VEGF expression in human bladder cancer cells was detected with immunohistochemistry. Endothelial cell growth was assessed after adding conditional medium. Results By immunohistochemistry, the positive cells were 89. 1/100 cells in control group, 90. 3/100 cells in ^125I-Na group, 10. 3 in ASODN group and 4, 3 in ^125I-ASODN group. The D value of each group was 0, 398±0. 031,0, 387 ± 0. 010,0, 298±0. 013 and 0. 148 ± 0. 011. Comparing ^125I-ASODN group and ASODN group with control and ^125I-Na group, difference was significant (P〈0, 05). Conclusion ^125I-ASODN can effectively suppress VEGF expression of human bladder cancer cells. It has good prospect in canti-angiogenesis of bladder carcinoma.
出处
《肿瘤防治研究》
CAS
CSCD
北大核心
2005年第10期632-633,647,共3页
Cancer Research on Prevention and Treatment
关键词
反义
寡核苷酸
膀胱癌
血管内皮生长因子
放射性核素
Antisense
Oligonucleotides
Bladder carcinoma
Vascular endothelial growth factor(VEGF)
Radionuclide