血管内皮生长因子在食管癌组织中的表达及其临床意义研究

Study on the Expression of VEGF in Human Esophageal Cancer Tissue

目的探讨食管癌组织中VEGF的表达水平与食管癌生物学特性的关系。方法应用免疫组化法分析人食管癌组织中VEGF的表达和微血管密度;应用RT-PCR法检测食管癌组织及人食管癌细胞株VEGFmRNA的表达。结果食管癌组织的VEGF阳性表达率为70.9%,VEGF在肿瘤组织的表达水平明显高于癌旁组织,其表达水平与肿瘤的病理学分级、肿瘤的浸润深度及淋巴结转移等行为有关。食管癌组织中微血管分布不均,血管密集区主要集中在癌灶边缘;MVD与肿瘤的分化程度和淋巴结转移相关;MVD与VEGF的表达之间存在正相关关系。食管癌组织的VEGFmRNA表达比癌旁组织增高,以VEGF189、VEGF165和VEGF121三种亚型为主。结论VEGF在食管癌组织中呈高表达状态,该现象与肿瘤的恶性程度、转移情况及微血管形成能力相关。

Objective To study the expression of VEGF in human esophageal cancer tissue and analysis the relationship between the expression of VEGF and the biological characteristics of esophageal cancer. Methods The microvessel density （MVD） and the expression of VEGF in human esophageal cancer tissue were studied by immunohistochemistry staining. The expression of VEGF in human esophageal cancer tissue and in human esophageal cancer cells （TE 1, TE-10） were determined by reverse transcriptasepolymerase chain reaction（RT PCR） method. Results The expression rate of VEGF in esophageal cancer tissues was 70. 9%. The expression level of VEGF in esophageal cancer tissue was higher than that in normal esophageal tissue. The expression level of VEGF also significantly correlated to pathologic grades,lymph node metastasis and invading depth of the tumors. MVD was also significantly associated with pathologic grades,lymph node metastasis of tumors. There were positive relationship between MVD and VEGF. The expressions of VEGF including VEGF189 .VEGF165 .VEGF121 were detected in human esophageal cancer tissues. Conclusion The development of esophageal cancer is related to higher expression of VEGF. There were relationship between over-expression of VEGF in esophageal cancer and grade, metastasis, MVD of esophageal cancer.