共刺激阻断剂与雷帕霉素联合应用对移植肾存活的影响

Influence of CTLA4Ig combined with rapamycin on the survival of renal allografts in rats

目的:探讨共刺激阻断剂CTLA4Ig与雷帕霉素(Rapamycin,RPM)联合应用对大鼠移植肾存活的影响。方法:肾移植大鼠分为对照组、CTLA4Ig组、RPM组和CTLA4Ig+RPM组,观察术后血肌酐和移植肾病理改变、移植肾存活时间。结果:与对照组相比,CTLA4Ig组、RPM组、CTLA4Ig+RPM组移植肾存活时间均显著延长(P<0.01),其中,CTLA4Ig+RPM组移植肾存活时间最长(76.5±17.1d),术后15天、30天该组血肌酐浓度最低;术后30天,仍然存活的CTLA4Ig组移植肾显示较多淋巴细胞浸润,CTLA4Ig+RPM组移植肾显示很少淋巴细胞浸润。结论:共刺激阻断剂与雷帕霉素联合应用可有效抑制排斥反应,显著延长大鼠移植肾存活时间。

Objective：To study the influence of Costimulatory Blockade CTLA4Ig combined with rapamycin（ RPM）on the survival of renal allografts in rats. Methods ：BN→Lewis rat kidney transplantation was perfomaed. The recipients were grouped into groupl （control）, group2 （ CTLA4Ig ）, group3 （ rapamycin, RPM） and group4 （CTLA4Ig ＋ RPM）. The survival of renal allografts , serum creatinine of the recipients and the pathological changes of the allografts were observed. Results：Significantly prolonged allografts survival time was seen in group 2,3 and 4 compared with control, with the longest in group 4. The lowest serum creatinine and fewest lymphocytes infiltrating grafts were also seen in group4. Conclusion ：Combined use of CTLA4Ig and rapamycin（RPM）may suppress renal allograft rejection effectively and promote further prolongation of the survival of renal allografts.