幽门螺杆菌感染与胆囊结石形成的相关性分析

Analyze the associativity between Helicobacter pylori infection and cholecystolithiasis

目的:研究和探讨幽门螺杆菌(Helicobacter pylori,Hp)与胆囊结石形成关系,寻找Hp在胆道中存在的证据,同时对胆道中HpDNA的检测方法进行研究和分析。方法:参照文献对120例胆囊结石患者胆汁、粘膜和胆石进行Hp培养,并以Ure A和cagA作引物进行扩增,对扩增产物进行分析。结果:120例样本检测均为阴性结果,培养无Hp生长,使用PCR方法未检测到Hp DNA及Hp CagA抗体。结论:(1)运用PCR方法在胆汁和胆石中检测HpDNA尚受到多种因素制约。(2)Hp可能经Oddi′s括约肌逆行的进入胆道但与胆囊结石并无明确相关性。

Objective ：To investigate the relationship of H pylori with cholecystolithiasis, to collect the evidence of Hp existing in biliary tract, and to study the detective method of Hp DNA in billiary tract. Methods ：Hp which existing in bile, mucosa and gallstone of 120 cholecystolithiasis patients were cultured according to the literature. Then they were amplified by the primer of Ure A and ca gA. Amplified products were analyzed. Results ：All of the 120 ,samples were negative. No Hp grew in the medium. No Hp DNA and Hp Cag A antibody were found with PCR method. Conclusion ： （ 1 ） It was restricted by many factors that Hp DNA in bile and gallstone wereexamined with PCR. （2） Hp maybe entered biliary tract regressively through Oddi＇s sphincter andhad no relationship with cholecystolithiasis.