丙型肝炎病毒不同片段抗体蛋白芯片的制备及评价

Preparation and evaluation of proteinchip for different HCV antibody detection

目的:制备丙型肝炎病毒(hepatitisvirusC,HCV)不同片段抗体蛋白芯片,并对其临床应用价值进行评价.方法:分别取HCV融合抗原及分片段抗原,点至醛基化玻片上,制成HCV不同片段抗体蛋白芯片.进行抗HCV(ELISA)试剂和RIBA试剂与蛋白芯片法的比较.结果:蛋白芯片的融合抗原检测与ELISA法相比,阴性符合率为93.8%,阳性符合率为97.1%.蛋白质芯片分片段检测与ELISA法相比,阴性符合率为96.8%,阳性符合率为97.1%.蛋白芯片的融合抗原检测与RIBA法相比,阴性符合率为96.9%,阳性符合率为98.5%.蛋白芯片分片段检测与RIBA法相比,阴性符合率为100%,阳性符合率为98.5%.结论:蛋白芯片法与RIBA试剂检测结果相比较,与RIBA试剂有良好的一致性(P<0.05)),证明其具有良好的检测准确率,并可以降低ELISA法的假阳性.

AIM： To prepare and evaluate the clinical application of protein-chip for different hepatitis virus C （HCV） antibodies. METHODS： Proteinchip for different HCV antibodies was established by coating chimeric antigen and the 4 segments of HCV antigens on the slide. The results of this method were compared with those of RIBA and ELISA. RESULTS： The negative result and the positive result of proteinchips of chimeric antigen accorded with ELISA were 93.8 % and 97. 1%, respectively. When it came to RIBA, the accordance rate was 96.9 % and 98.5%, respectively. The accordance rates of negative and positive results of proteinchip of the 4 segments of HCV antigens with ELISA were 96.8% and 97. 1%, respectively, while they turned out to be 100% and 98.5% with RIBA. CONCLUSION： The proteinchip for different HCV antibodies has a high accordance rate with RIBA （ P 〈 0.05）. Proteinchip for different HCV antibodies has high accuracy and can decrease the false positive rate.