摘要
目的:制备丙型肝炎病毒(hepatitisvirusC,HCV)不同片段抗体蛋白芯片,并对其临床应用价值进行评价.方法:分别取HCV融合抗原及分片段抗原,点至醛基化玻片上,制成HCV不同片段抗体蛋白芯片.进行抗HCV(ELISA)试剂和RIBA试剂与蛋白芯片法的比较.结果:蛋白芯片的融合抗原检测与ELISA法相比,阴性符合率为93.8%,阳性符合率为97.1%.蛋白质芯片分片段检测与ELISA法相比,阴性符合率为96.8%,阳性符合率为97.1%.蛋白芯片的融合抗原检测与RIBA法相比,阴性符合率为96.9%,阳性符合率为98.5%.蛋白芯片分片段检测与RIBA法相比,阴性符合率为100%,阳性符合率为98.5%.结论:蛋白芯片法与RIBA试剂检测结果相比较,与RIBA试剂有良好的一致性(P<0.05)),证明其具有良好的检测准确率,并可以降低ELISA法的假阳性.
AIM: To prepare and evaluate the clinical application of protein-chip for different hepatitis virus C (HCV) antibodies. METHODS: Proteinchip for different HCV antibodies was established by coating chimeric antigen and the 4 segments of HCV antigens on the slide. The results of this method were compared with those of RIBA and ELISA. RESULTS: The negative result and the positive result of proteinchips of chimeric antigen accorded with ELISA were 93.8 % and 97. 1%, respectively. When it came to RIBA, the accordance rate was 96.9 % and 98.5%, respectively. The accordance rates of negative and positive results of proteinchip of the 4 segments of HCV antigens with ELISA were 96.8% and 97. 1%, respectively, while they turned out to be 100% and 98.5% with RIBA. CONCLUSION: The proteinchip for different HCV antibodies has a high accordance rate with RIBA ( P 〈 0.05). Proteinchip for different HCV antibodies has high accuracy and can decrease the false positive rate.
出处
《第四军医大学学报》
CAS
北大核心
2005年第20期1858-1860,共3页
Journal of the Fourth Military Medical University
基金
陕西省科学技术研究发展计划项目(2003K10G62)
关键词
肝炎病毒
蛋白质陈列分析
抗体
hepacivirus
protein array analysis
antibodies