巨细胞病毒抗原分支肽的合成与鉴定

Synthesis and identification of CMV branched peptide

目的:探讨人巨细胞病毒(HCMV)抗原分支肽人工合成方法.方法:用固相合成方法分别合成HCMV抗原表位的线性多肽和分支多抗原肽,经HPLC进行纯化后质谱检验其分子质量,并用质控血清鉴定抗原活性.结果:合成的线性肽分子质量为793.87ku,八分支肽的分子质量为7127.77ku,均与其理论分子质量相符,分支肽经ELISA初步鉴定对质控血清具有较好的分辨效果.结论:HCMV抗原分支肽的人工合成大大简化了以往HCMV抗原的制备方法,且制备成本低,无潜在生物危害.

AIM： To investigate the synthesis of human cytomegalovirus antigenic branched peptide. METHODS： The linear peptide and branched multiple peptide with human cytomegalovirus＇ antigenic epitope were synthesized with solid phase chemistry respectively and purified by HPLC. The two peptides＇ molecular masses were obtained by mass spectrum and then identified with quality control serums. RESULTS： The molecular mass of the linear peptide was 793.8 and that of branched multiple antigenic peptide was 7127.77, both consistent with the theoretic molecular mass. The branched peptide differentiated quality control serums properly through ELISA. CONCLUSION： The synthesis of cytomegalovirus antigenic branched peptide greatly facilitates the previous way for the preparation of human cytomegalovirus antigen and has the advantage of low cost and no potential biohazard.