静脉血栓形成对大鼠非血栓段静脉血管内皮细胞的影响

Effects of venous thrombosis on endothelial cells in remote non-thrombosed vein

目的研究静脉血栓对非血栓段静脉内皮细胞的损伤作用。方法90只SD大鼠随机分为血栓、对照、正常3组:血栓组在左髂静脉以结扎法建立血栓模型;对照组开腹显露髂动静脉模拟同等程度创伤;正常组直接取材送电镜、免疫组化、凋亡和Westernblotting研究。结果血栓组非血栓段内皮细胞形态、功能发生改变;血栓组与对照组相比术后1、2、4、7dFITC染色的凋亡率P值分别<0.00、0.05、0.01、0.00、术后1、2、4、7dPropidiumIodide染色的凋亡率,P值均<0.05,差异有统计学意义。结论局部深静脉血栓可以导致其他部位静脉内皮细胞损伤,使这些部位发生血栓的危险性增加。

Objective To investigate the functional changes of endothelial cells （EC） in non-thrombosis vein segment （ECNT） in rats with experimental venous thrombosis （VT）. Method Rats were divided into normal, control, and thrombosis group caused by legating a portion of iliac vein with 30 animals in each group. Endothelium was sampled from superhepatic IVC. Samples were studied by electroscopy, immunohistochemistry, and Western blotting for apoptosis. Results Morphological and functional changes of the ECs were observed in sampled superhepatic IVC. Compared with normal and control group the apoptosis rates（ P 〈0. 00,0. 05,0. 01,0. 00 at 1,2,4,7 days post-op） of ECs in thrombosis group at different times post-op were significantly increased. Conclusion Venous thrombosis leads to the ECs injury in remote non-thrombosed veins.