拟南芥中一个类似钙调素蛋白的基因结构及其缺磷、缺钾诱导表达

The Structure and Phosphorus or Potassium Deficiency Induced Ex- pression of a Calmodulin-like Protein Gene in Arabidopsis

通过拟南芥芯片杂交分析发现推测的钙调素基因(GenBankaccessionNo.At1g76650)与低磷胁迫有关。对该基因的结构研究确认了该基因编码一个含有三个EF-hand结构域的类似钙调素的蛋白,Northern检测表明该基因在缺钾、缺磷条件下诱导表达,但在缺氮、高盐等胁迫条件下不受影响。经RT-PCR和启动子融合GUS转基因植株的组织化学染色分析,表明该基因在拟南芥中为全株表达,但各器官中表达丰度不尽相同。

According to our previous microarray analysis, we found a putative calmodulin gene related to Pi deficiency and designated AtPsiCaM （Arabidopsis Pi-starvation-induced CAM）. Resuits of structural analysis indicate that AtPsiCaM has three conserved EF-hands motif and belongs to calmodulin-like proteins family （Figs. 1-3）. Northern blot analysis revealed that this gene could be induced by potassium and phosphate deficiency and not by potassium deficiency or high salinity （Fig.4）. The results of RT-PCR and GUS histochemical staining assays of the AtPsiCaM promoter：：GUS transgenic plants showed that this gene can be expressed in all tissues to different expression levels （Figs.5, 6）.