新生小鼠吸入高氧视网膜的血管发育与胶质纤维酸性蛋白表达的变化

Relationship between expression of glial fibrillary acidic protein and retinal vascular development in hyperoxia mouse

目的:研究新生小鼠吸入高氧视网膜病(ROP)过程中胶质纤维酸性蛋白(GFAP)表达的变化规律,探讨M黮ler细胞与血管发育的关系.方法:实验用生后7d(P7)C57BL/6J小鼠.ROP模型组在75%氧环境中饲养.在P9、P12、P14、P17和P21等不同时间取眼球,分别用ADP酶组织化学染色方法显示视网膜血管发育及免疫组织化学方法标记视网膜GFAP的表达.结果:小鼠视网膜血管生后开始发育,P21基本成熟;吸高氧后从P14开始出现新生血管,在P17～P21达到高峰.模型鼠视网膜M黮ler细胞从P14起内侧突起开始表达GFAP,到P21已遍布细胞全层.结论:高氧可导致视网膜发育晚期大量血管新生,且与GFAP表达的变化规律相一致,提示M黮ler细胞与视网膜血管发育关系密切.

Objective： To investigate the expression of glial fibrillary acidic protein （GFAP） in the retinopathy of prernaturity （ROP） model mice, and explore the relationship between Mtiller cells and retinal vascular development. Methods： One-week-old C57BL/6J mice were exposed to 75% oxygen for 5 days and then to room air. Mice of postnatal day 9 （Pg）, P14, P17, and P21 were perfused and eyes were enucleated. ADPase staining was used to delineate retinal vascular development and imrnunohistochemistry was used to study the expression of GFAP. Results： The retinal vasculature in mice was fully developed around P21. Hyperoxia-induced neovascularization occurred on P14 and reached maximum neovascular response from P17 to P21. Müller cells began to express GFAP from P14, and on P21 all Müller cells were GFAP positive. Conclusion： Hyperoxia induced neovascularization is in accordance with the change of GFAP expression, suggesting that there is a close relationship between Müller cell and retinal vascular development.