脑缺血再灌注损伤后神经细胞增殖相关因子基因的表达

Gene expression of nerve cell proliferation associated factors following cerebral ischemia reperfusion in rats

目的:观察大鼠脑缺血再灌注后神经细胞巢蛋白、干细胞因子(SCF)和神经细胞粘附分子(NCAM)基因的表达。方法:成年SD大鼠,以线栓法建立大脑中动脉缺血再灌注模型,随机分为缺血1.5 h再灌注2 h^14 d组和假手术组。原位杂交技术检测脑缺血再灌注后脑组织巢蛋白SCF和NCAM mRNA的表达。结果:脑缺血再灌注后,巢蛋白mRNA表达在大部分时相点,均明显高于假手术组。SCF mRNA的表达除早期时相点外,均明显高于假手术组。NCAM mRNA于再灌注2 h后开始表达,12 h^1 d达高峰,7 d后逐渐减少,14 d降至假手术组水平。结论:脑缺血再灌注后SCF mRNA表达可能具有促进神经干细胞增殖作用,NCAM表达可能参与了损伤后脑组织的修复过程。

Objective： To study the expressions of Nestin, stem cell factor （SCF） and neural cell adhesion molecule （NCAM） in neurons after ischemia reperfusion injury in rat brain. Methods： Thirty-six adult female SD rats were induced by intraluminal middie cerebral artery （MCA） occlusion for 1.5 h and reperfusion for 2 h, 6 h, 12 h, 1 d, 2 d, 3 d,7 d and 14 d [treated groups and sham-operated group （n：4）3 with a nylon monofilament suture. In situ hybridization was used to examine the expression of Nestin, SCF and NCAM mRNA. Results： Nestin mRNA expression in cortex and striatum increased markedly in ischemic hemisphere at most times compared to sham-operated group. SCF mRNA expression in cortex and striatum increased significantly in ischemic hemisphere except for early times compared to sham-operated group. NCAM mRNA expression was observed after reperfusion 2 h, peaked at reperfusion 12 h in cortex and 1 d in striaum, and decreased to the basic level of sham-operated group at reperfusion 14 d Conclusion： SCF expression may enhance neural stem cells proliferation following ischemia reperfusion. The increasing NCAM expression may play an important role in the repair of injured brain tissue.