摘要
鸡胚胎是一种被广泛应用于发育生物学研究的实验材料。在以鸡胚胎为动物模型的各项研究工作 中,心率常被看作是一个很重要的反应胚胎生理活动指标。本文详细介绍了一种侵入性的心电记录新方法,在 正常的生理条件下通过记录鸡胚胎心电的变化来监测心率。首先在蛋壳上钻孔,将电极插入蛋内,然后通过放 大器放大,A／D板转换,将心电信号输入电脑进行分析处理,提取与心率相关的信息。这种记录方式对胚胎损 伤较小,不影响胚胎的正常发育;具有灵敏度高,操作简单,容易掌握等特点。
The chick embryo is a widely used experimental animal in developmental biology research. Heart rate （HR） is usually considered to be an important physiological index reflecting the embryo＇s natural activity. The paper introduces in detail a new invasive method for heart rate recording. With wire electrodes implanted by drilling small holes in the egg＇s shell, signals are amplified and digitised using an A/D converter. The heart rate （HR） could be measured under normal physiological conditions, and thus the normal embryonic development is not significantly affected by the recording procedure. In addition, the signals are large in amplitude and easy to manipulate.
关键词
鸡胚胎
心率
侵入性记录法
Chick embryo
Heart-rate （HR）
Invasive recording
