联合应用NGF和GM1对大鼠坐骨神经损伤后再生及功能恢复的影响

Co-application of NGF and GM1 for regeneration and functional recovery of injuried sciatic nerves in rats

目的:探讨联合应用神经生长因子(NGF)和神经节苷脂(GM1)对大鼠坐骨神经损伤后再生及功能恢复的影响。方法:选用SD大鼠96只,分为对照组、NGF组、GM1组和NGF+GM1组,将大鼠坐骨神经造成5mm的缺损,术中将远近神经断端纳入硅胶管内局部滴加药物、术后于大鼠损伤侧小腿肌注药物。术后行坐骨神经功能指数(SFI)评定及传导速度(NCV)检测;电镜下观察再生坐骨神经纤维数量和髓鞘厚度的变化。结果:术后4、6周时,NGF组和GM1组SFI、NCV及再生坐骨神经纤维数量及髓鞘厚度均明显高于对照组(P均<0.01);NGF+GM1组各指标均高于对照组、NGF组和GM1组(P均<0.01);而在术后8周时,NGF+GM1组、NGF组、GM1组各指标均高于对照组(P<0.01),但各实验组之间差异无统计学意义(P均>0.05)。结论:①GM1能够介导NGF促进坐骨神经损伤后再生及功能恢复,表现出良好的生物学效应,并且效果优于单用NGF,GM1;②与单用NGF或GM1相比,NGF+GM1能更早期的在坐骨神经损伤后发挥再生及功能恢复的作用。

Objective ：To investigate the effect of co-application of NGF and GM on the regeneration and functional recovery of transected sciatic nerves in rats. Methods：ninety-six SD rats were randomly divided into the normal saline group,NGF group,GM1 group and NGF ＋ GM1 group. The sciatic nerves were incised for 5 mm defect. Saline , NGF, GM1 and NGF＋GM1 were injected into tube immediately at the end of the operation and injected into the muscle of injured leg once a day. The sciatic nerve function index （SFI）,nerve conduction velocity （NCV）,the account of the number of nerve fibers of the injured sciatic nerves and the thickness of myelin sheath were measured at the 4th, 6th,Sth weeks postinjury. Results：At weeks 4 and 6, the SFI and NCV were significantly higher in NGF ＋ GM1 group than in other groups;the number of sciatic nerve fibers and the thickness of myelin sheath were increased significantly in groups 2,3 and 4 than in group 1. In the 8th week,group 2,3 and 4 showed significant increase in the SFI, NCV,number of sciatic nerve fibers and thickness of myelin sheath compared with group 1 ;there were no significant differences among group 2,3 and 4. Conclusion：The GM1 can lead the NGF to enhance peripheral nerve regeneration and functional recovery;compared with only use of NGF or GM 1,co-application of NGF and GM 1 can express a better and earlier biology effect.