EGFR反义脱氧寡核苷酸硫代修饰片段对人肝癌细胞中血管内皮生长因子mRNA表达的研究

Phosphorothioate oligodeoxynucleotides of epidermal growth factor receptor on the regulation of vascular endothelial growth factor gene expression in human hepatoma cells

目的:探讨表皮生长因子受体(epidermal growth factorr eceptor,EGFR)反义脱氧寡核苷酸硫代修饰片段对人肝癌细胞血管内皮生长因子(vascular endothelial growth factor,VEGF)基因表达的影响。方法:应用逆转录聚合酶链反应(RTPCR)法,以次黄嘌呤磷酸核糖转移酶(hypoxanthine phosphoribosyl-transferase,HPRT)为内标,分析从1.6～3.2μmol/L浓度的EGFR反义脱氧寡核苷酸硫代修饰片段在84h时对人肝癌细胞VEGF基因mRNA表达水平的影响。结果:EGFR反义脱氧寡核苷酸硫代修饰片段有明显的抑制人肝癌细胞表达VEGF的作用,且这种抑制作用随着EGFR反义脱氧寡核苷酸硫代修饰片段浓度的增高而加强。结论:EGFR反义脱氧寡核苷酸硫代修饰片段能抑制人肝癌细胞VEGF基因的表达,为肝癌的抗血管治疗提供了新的途径和方法。

Objective： To investigate the effect of phosphorothioate oligodeoxynucleotides （S- ODNs） of epidermal growth factor receptor （EGFR） on the regulation of vascular endothelial growth factor （VEGF） gene expression in human hepatocellular carcinoma cells. Methods： Treated with concentration range of 1.6-3.2μmol/L S-ODNs in culture medium,the relative VEGF mRNA level at 84th hour in human hepatoma cells was evaluated by reverse-transcriptase polymerase chain reaction （RT-PCR） with hypoxanthine phosphoribosyhransferase （HPRT） as an internal control standard. Result： The expression of VEGF gene mRNA was inhibited obviously by S-ODNs, and the inhibition was dependent upon the dose. Conclusion： SODNs can regulate and inhibit the expression of VEGF gene in human hepatocellular carcinoma cells, which might contribute to the development of a new therapeutic strategy for anti-angiogenesis.