采用RNAi技术抑制PeroxiredoxinⅠ的表达

Inhibited Expression of PrxⅠ in NIH3T3 Cell by RNA Interference

Peroxiredoxin Ⅰ(PrxⅠ) is involved in many important cellular progresses such as cell proliferation and differentiation. To further elucidate its roles in ionizing radiation, eukaryotic expression vector containing siRNA targeting mRNA of peroxiredoxin 1 gene was constructed and transected into NIH3T3 cell line following sequencing.Western blot analysis showed that this siRNA significantly inhibited PrxⅠ expression by 65% at protein level; cell proliferation experiment demonstrated that the double proliferation time of NIH 3T3PrxⅠ cells was postponed for 1.8 hours as compared with control group. MTT results showed that 12 and 48 hours after irradiation the survival rate of NIH 3T3PrxⅠ was dramatically decreased when comparing with sham-irradiated group.These results suggested that peroxiredoxin 1 has radiation-cytoprotection role.

Peroxiredoxin Ⅰ （ Prx Ⅰ ） is involved in many important cellular progresses such as cell proliferation and differentiation. To further elucidate its roles in ionizing radiation, eukaryotic expression vector containing siRNA targeting mRNA of peroxiredoxin 1 gene was constructed and transected into NIH3T3 cell line following sequencing. Western blot analysis showed that this siRNA significantly inhibited Prx Ⅰ expression by 65 % at protein level; cell proliferation experiment demonstrated that the double proliferation time of NIH 3T3Prx Ⅰ cells was postponed for 1.8 hours as compared with control group. MTF results showed that 12 and 48 hours after irradiation the survival rate of NIH 3T3Prx Ⅰ was dramatically decreased when comparing with shamirradiated group. These results suggested that peroxiredoxin 1 has radiation-cytoprotection role.