鼻咽癌相关肿瘤抗原基因的筛选及鉴定

Screening and Identification of Human Nasopharyngeal Carcinoma Associated Antigens

目的通过筛选鼻咽癌肿瘤抗原,为鼻咽癌提供一个早期诊断指标;同时为进一步研究鼻咽癌的免疫治疗奠定基础。方法应用鼻咽癌细胞株异种免疫血清,采用血清学筛选重组cDNA表达文库(SEREX)技术对鼻咽癌cDNA表达文库进行筛选,对阳性克隆测序并进行生物信息学分析。W estern B lot检测鼻咽癌及正常人血清中阳性克隆的抗体。结果共筛选出18个阳性克隆,其中包括M AGE基因、CREM基因、2β-微球蛋白以及核糖体蛋白等已知基因或表达序列标签(EST),另外有6个基因或EST在G enB ank数据库中没有同源性,可能是新基因或EST。选取NPC-14、NPC-15、NPC-18克隆进行鼻咽癌患者及正常人群血清的检测,结果显示鼻咽癌患者的阳性率明显高于正常人群。结论所获得的未知基因产物有可能成为早期诊断鼻咽癌的血清学标志物。

Objective The purpose of this study was to provide an informative marker for clinical immunopathological diagnosis of nasopharyngeal carcinoma （NPC） and to lay a foundation for furture study of immunotherapy. Methods The NPC eDNA expression library was screened by serological analysis of recombinant eDNA expression libraries （SEREX） technology using xenogenic immune sera. The positive nucleotide sequences of eDNA inserts were determined and analysed with DNASIS and BLAST software in EMBL and GenBank. Results Eighteen positive clones encoding antigen genes were obtained after immunoscreening. These antigen genes included known genes, such as MAGE gene, CREM gene, β2-microglobulin, Ribosomal Protein, et al, and unknown genes or ESTs in GenBank. To characterize the expression profile of these genes, the antibodies in sera from 40 nasopharyngeal carcinoma patients and 40 healthy donors were assayed with three antigens to screen specific and relative serum markers for nasopharyngeal carcinoma. The results showed that the positive rates for nasopharyngeal carcinoma patients were higher than those for healthy donors. Conclusion These results indicate that some of the antigens may be related to nasopharyngeal carcinoma and may be valuable tumor markers in the diagnosis of nasopharyngeal carcinoma.