血小板源生长因子受体结构域切除对肺血管平滑肌细胞增殖和c-sis基因表达的影响

Effect of Truncated PDGF-Α Receptor on Proliferation and Expression of c-sis mRNA of Vascular Smooth Muscular Cells of Pulmonary Artery

目的探讨从血小板源生长因子α(PDGF-α)受体水平阻断对肺血管平滑肌细胞增殖的影响。方法在肺动脉平滑肌细胞(V SM C)培养液中分别给予不同剂量的受体结构域切除的PDGF-α受体腺病毒重组体A d5CM V-PaR tr(ACP),对细胞不同时相的增殖曲线和c-s is原癌基因表达进行测定。结果5 m l/L、10 m l/L剂量ACP对细胞增殖有明显抑制作用。在5 m l/L ACP作用下,加入PDGF-BB不能促进V SM C的增殖。不同剂量的ACP可使c-s is mRNA的表达上调。5 m l/L ACP作用下,加入PDGF-BB可下调c-s is mRNA的表达。结论5 m l/L、10 m l/L ACP能明显抑制肺V SM C的增殖,使c-s is mRNA的表达上调。

Objective To investigate the effect of truncated PDGF-α receptor on proliferation and expression of c-sis mRNA of pulmonary artery vascular smooth muscular cells （VSMC）. Methods Tissue mass culture was done to get vascular smooth muscular cells of pulmonary artery. Different concentrations of truncated PDGF-α receptor and adenoviral recombined body （AdSCMV-PαRtr, ACP） were added into the cultures. The VSMC proliferation curves were obtained using MTT test. The expression of c-sis mRNA was detected by RT-PCR. Results ACP at 5 ml/L and 10 ml/L concentrations restrained the proliferation of VSMC apparently with the peak of cell growth being attenuated or eliminated. The curve presented ascending tendency only after 7 to 9 days. Affected by 5 ml/L ACP, PDGF-BB exerted no significant accelerating effect on the proliferation of VSMC. The expression of c-sis mRNA was up-regulated under the effect of ACP. Affected by 5 ml/L ACP and PDGF-BB, the expression of c-sis mRNA was down-regulated. Conclusion ACP at 5 ml/L and 10 ml/L concentration is a powerful inhibitor of cellular proliferation for pulmonary artery VSMC. It can increase c-sis mRNA expression significantly and the increase seems to be ACP dosage dependent.