肺炎球菌多糖疫苗对老龄大鼠肺组织-防御素-2表达的影响

Study of β-defensin-2 Gene Expression in the Pulmonary Tissue of the Older Rat Stimulated by Pneumococcal Vaccine Polyvalent

目的研究肺炎球菌多糖疫苗(纽莫法)对老龄大鼠肺组织β-防御素-2基因表达的影响.方法将18～22月的老龄大鼠随机分为实验组与对照组,实验组给予纽莫法0.2 ml/kg腹腔注射,对照组给予等量生理盐水腹腔注射.24 h后处死,取肺组织,提取总RNA,以β-actin为内参照,采用半定量逆转录聚合酶链反应(RT-PCR)检测肺组织rBD-2基因表达水平.结果实验组老龄大鼠24 h后rBD-2基因表达水平为0.500±0.126,对照组为0.209±0.138,两组间差异有统计学意义(P<0.05).结论纽莫法可上调老龄鼠肺组织β-防御素-2基因的表达,增强机体局部免疫力.

Objective To investigate the effect of pneumococcal vaccine polyvalent （pneumovax23） on β-defensin-2 gene expression in older rats. Methods Twenty older male Wistar rats（18-22 months） weighing （600±50）g were randomly allocated into two groups. The rats of experimental group were intraperitoneally injected with pneumovax23 0.2 ml/kg; the rats of control group were intraperitoneally injected with equivalent normal saline. Semiquantitive reverse transcription polymerase chain reaction （RT-PCR）was performed to detect β-defensin-2 mRNA expression; β-actin was used as the internal standard. Results The expression of β-defensin-2 gene increased in the experimental group, as compared with that in the control group. Conclusion Pneumovax23 can upregulate the expression of rBD-2 gene in the lung tissue and enhance the body local immunity. These experimental data indicate that Pneumovax23 can be used to enhance β-defensin-2 and hence protect the old rats from pneumococci.