攀枝花地区野生番石榴叶不同提取物降血糖作用研究

Study on the Hypoglycemic Activity of Different Extracts of Wild Psidium guajava Leaves in Panzhihua Area

目的研究攀枝花地区野生番石榴叶的水提取物、650 m l/L和950 m l/L食用乙醇提取物的降血糖作用。方法雄性昆明种小鼠,诱导葡萄糖性、肾上腺素性和链脲佐菌素性高血糖小鼠模型,比较三种提取物对血糖水平的影响;观察脏器变化,比较体重及肝脏、肾脏、脾脏和胰腺的脏器指数的变化。结果三种提取物能不同程度降低小鼠外源性高血糖、拮抗应激性血糖升高;三种提取物可明显降低糖尿病小鼠血糖水平,水提物、650 m l/L和950m l/L食用乙醇提取物,降糖效率分别为36.3%、33.5%和31.3%。三种提取物中,水提物对小鼠的生长影响较小。结论攀枝花地区野生番石榴叶的水提取物、650 m l/L和950 m l/L食用乙醇提取物具有不同程度的降血糖作用。

Objective To illuminate the role of water-soluble, 650 ml/L edible alcohol and 950 ml/L edible alcohol-soluble extracts of wild Psidium guajava leaves in Panzhihua Area in decreasing blood glucose. Methods High-level blood glucose models were made by use of male Kunming mice given intraperitoneal injection of glucose, subcutaneous injection of adrenaline and intraperitoneal injection of streptozotocin （STZ）, respectively. Blood glucose concentration was measured after oral administration （gastrogavage） of the soluble extracts of Psidium guajava leaves, respectively. Body weight and organ morphology were observed, and organ index was obtained. All available indexes were statistically analyzed in comparing the study groups and control group. Results The three extracts resisted the rise of blood glucose level induced by exogenous glucose and adrenaline to various degrees. The extracts of water, 650 ml/L alcohol and 950 ml/L alcohol significantly decreased the blood glucose level in STZ-induced diabetic mice by 36.3%, 33.5% and 31.3% respectively. Furthermore, among three extracts, water-soluble extract showed little influence on the growth of mice. Conclusion The water-soluble, 650 ml/L edible alcohol and 950 ml/L edible alcohol-soluble extracts of wild Psidium guajava leaves in Panzhihua area may have different hypoglycemic potential.