自体成骨细胞与脱钙骨复合移植修复兔桡骨缺损的实验研究

Experimental Study on Repair of Rabbit Radial Defects by Composite Grafting of Osteoblast and Decalcified Bone Matrix(DBM)

目的研究成骨细胞与DBM复合后的成骨能力,为临床修复骨缺损提供一条新途径。方法20只新西兰大白兔随机分为4组,每组5只,右侧植入成骨细胞与DBM复合物,左侧植入DBM,分别在2、4、8、12周做X线、组织学切片、微量元素测定等观察,结果进行统计学分析。结果纯化后的细胞具有成骨细胞的形态结构和生物学特性,复合物修复骨缺损的速度和质量明显优于对照组。结论成骨细胞与DBM能很好地复合,制成有成骨活性的复合体,在修复骨缺损过程中起到诱导成骨和传导成骨的作用。

Objective To study the osteogenic potential of composite grafting of osteoblast and DBM and find a new model of repairing segmental bony defects. Methods Twenty rabbits were divided randomly into 4 groups. Compounds were implanted into the right radial bone defects and DBM were implanted into the left defects as control. Examinations were performed at different time after operation as follows： radiographic examination, histological observation and trace element content determination. Results The morphological and biological characteristics of the cultured stromal cells were similar to osteoblasts. The quantity of new bone formation and speed of repairing bone defect in osteoblast/DBM groups were superior to those in DBM groups. Conclusion The results showed that cultured osteoblasts were capable of repopulation on DBM in vitro and performed important functions in inductive osteogenesis and conductive osteogenesis in vivo.